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Titolo:
QUANTIFICATION OF ION-TRANSPORT IN PERFUSED RAT-HEART - CS-133(+) AS AN NMR ACTIVE K+ ANALOG
Autore:
SCHORNACK PA; SONG SK; LING CS; HOTCHKISS R; ACKERMAN JJH;
Indirizzi:
WASHINGTON UNIV,DEPT CHEM,CAMPUS BOX 1134,1 BROOKINGS DR ST LOUIS MO 63130 WASHINGTON UNIV,SCH MED,DEPT ANESTHESIOL ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT MED ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT RADIOL ST LOUIS MO 63110 MONSANTO CO ST LOUIS MO 63141
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 5, volume: 41, anno: 1997,
pagine: 1618 - 1634
SICI:
0363-6143(1997)41:5<1618:QOIIPR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PIG VENTRICULAR MYOCYTES; CARDIAC PURKINJE-FIBERS; POLYETHYLENE-GLYCOL; EXTRACELLULAR-SPACE; MYOCARDIAL INJURY; MEMBRANE; EXCHANGE; TISSUE; CESIUM; CELLS;
Keywords:
NUCLEAR MAGNETIC RESONANCE; CESIUM; MEMBRANE TRANSPORT; TRANSPORT KINETICS; SODIUM PUMP; TRANSVERSE RELAXATION; LONGITUDINAL RELAXATION; INDUCTIVELY COUPLED PLASMA MASS SPECTROSCOPY; OUABAIN; COMPARTMENTAL MODELING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
P.A. Schornack et al., "QUANTIFICATION OF ION-TRANSPORT IN PERFUSED RAT-HEART - CS-133(+) AS AN NMR ACTIVE K+ ANALOG", American journal of physiology. Cell physiology, 41(5), 1997, pp. 1618-1634

Abstract

Proper ion balance between intra- and extracellular compartments is necessary for normal physiological function. Conversely, alterations inmembrane ion transport occur in numerous pathological states. As a noninvasive, nondestructive spectroscopic technique, nuclear magnetic resonance (NMR) offers a powerful approach to the study of ion balance in intact biological systems. Unfortunately, rare NMR active nuclides that are isotopes of the 100% naturally abundant Na-23(+) and K-39(+) are not available for tracer kinetic studies of Na+ and K+ transport. However, Cs+ is a biologically active analog of K+, and the 100% naturally abundant NMR active Cs-133(+) nuclide can be employed to examine K transport (Davis, D. G., E. Murphy, and R. E. London. Biochemistry 27: 3547-3551, 1988). The distinguishing feature of Cs-133(+) is that it naturally gives two separate well resolved NMR resonances for intra-and extracellular Cs-133(+), permitting study of the time course changes of either of these compartments independent of the other. In this report, the experimental procedures and compartmental modeling formalism are developed that allow quantitative analysis of Cs+ membrane transport in the perfused rat heart. Intracellular Cs-133(+) is shown to be 100% visible by solution-state NMR methods and its influx transport to be markedly inhibited by ouabain, a confirmation of findings previously reported by others. Intracellular Cs-133(+) spin-lattice and spin-spin relaxation times at 7 T were determined to be 2.1 +/- 0.3 (SD) s(It = 8) and 0.065 +/- 0.007 (SD)s (n = 8), respectively, for T-1 andT-2. The rate constant for Na+-K+-ATPase pump dominated intracellularinflux was measured to be 0.25 +/- 0.07 (SD) min(-1) (n = 27) and that for efflux 0.005 +/- 0.001 (SD) min(-1) (n = 14). The rate constant for Cs-133(+) equilibration in the extracellular space at supraphysiological perfusate flow rate (20 ml/min) was found to be 4.6 +/- 0.9 (SD) min(-1) (n = 20). Thus extracellular diffusion limitations do not dominate the Cs-133(+) transport measurements.

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Documento generato il 17/01/21 alle ore 18:09:53