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Titolo:
NONCOORDINATE REGULATION OF EPITHELIAL NA CHANNEL AND NA PUMP SUBUNITMESSENGER-RNAS IN KIDNEY AND COLON BY ALDOSTERONE
Autore:
ESCOUBET B; COUREAU C; BONVALET JP; FARMAN N;
Indirizzi:
UNIV PARIS 07,INSERM,U426,FAC MED XAVIER BICHAT,BP 416 F-75870 PARIS 18 FRANCE UNIV PARIS 07,FAC MED XAVIER BICHAT,U246 PARIS FRANCE UNIV PARIS 07,FAC MED XAVIER BICHAT,DEPT PHYSIOL PARIS FRANCE UNIV PARIS 07,FAC MED XAVIER BICHAT,INSERM,INST FEDERAT RECH CELLULESEPITHELIALES PARIS FRANCE
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 5, volume: 41, anno: 1997,
pagine: 1482 - 1491
SICI:
0363-6143(1997)41:5<1482:NROENC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICAL COLLECTING TUBULE; ATPASE MESSENGER-RNA; RAT DISTAL COLON; SODIUM-TRANSPORT; CELL SODIUM; K-ATPASE; EXPRESSION; NA,K-ATPASE; STEROIDS; ALPHA-1;
Keywords:
SODIUM TRANSPORT; GENE; RAT; SODIUM PUMP; SUBUNITS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
B. Escoubet et al., "NONCOORDINATE REGULATION OF EPITHELIAL NA CHANNEL AND NA PUMP SUBUNITMESSENGER-RNAS IN KIDNEY AND COLON BY ALDOSTERONE", American journal of physiology. Cell physiology, 41(5), 1997, pp. 1482-1491

Abstract

Distal colon and renal cortical collecting ducts are major effecters of aldosterone-dependent Na homeostasis. Na is absorbed by entry through an apical amiloride-sensitive Na channel and extruded by Na-K-ATPase at the basolateral membrane. Using a ribonuclease protection assay, we studied, in vivo, aldosterone regulation of alpha-, beta-, gamma-subunits of the rat epithelial Na channel (rENaC) and alpha(1)- and beta(1)-subunits of Na-K-ATPase. In the kidney, Na-K-ATPase mRNAs were also assayed over discrete tubular segments by in situ hybridization. In rat colon, all three rENaC mRNAs were decreased by adrenalectomy, witha major effect on beta- and gamma-subunits, and were restored with 7 days, but not 2 days, of aldosterone treatment; in the kidney, however, only a-transcripts varied. Na-K-ATPase alpha(1)- and beta(1)-subunitmRNAs in both organs were not (in the case of the beta(1)-subunit) orwere mildly (in the case of the alpha(1)-subunit) affected after adrenalectomy. Our conclusions are as follows: 1) Transcripts of rENaC andNa-K-ATPase subunits are not coordinately regulated by aldosterone invivo; i.e., modulation involves mainly the Na channel, not Na-K-ATPase; the effect is not of comparable magnitude on each subunit mRNA and differs between tissues. 2) The delay of the aldosterone effect on transcripts is much longer than that required to restore normal Na transport in adrenalectomized rats, indicating that rENaC and Na-K-ATPase subunit transcript levels may depend on unidentified early aldosterone-induced proteins.

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Documento generato il 28/11/20 alle ore 21:38:52