Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ABNORMALITIES IN FUNCTIONAL-DEVELOPMENT OF THE SERTOLI CELLS IN RATS TREATED NEONATALLY WITH DIETHYLSTILBESTROL - A POSSIBLE ROLE FOR ESTROGENS IN SERTOLI-CELL DEVELOPMENT
Autore:
SHARPE RM; ATANASSOVA N; MCKINNELL C; PARTE P; TURNER KJ; FISHER JS; KERR JB; GROOME NP; MACPHERSON S; MILLAR MR; SAUNDERS PTK;
Indirizzi:
MRC,REPROD BIOL UNIT,CTR REPROD BIOL,37 CHALMERS ST EDINBURGH EH3 9EWMIDLOTHIAN SCOTLAND MRC,REPROD BIOL UNIT,CTR REPROD BIOL EDINBURGH EH3 9EW MIDLOTHIAN SCOTLAND BULGARIAN ACAD SCI,INST EXPT MORPHOL & ANTHROPOL BU-1113 SOFIA BULGARIA MONASH UNIV,DEPT ANAT CLAYTON VIC 3168 AUSTRALIA OXFORD BROOKES UNIV,SCH BIOL & MOL SCI OXFORD OX3 0BP ENGLAND
Titolo Testata:
Biology of reproduction
fascicolo: 5, volume: 59, anno: 1998,
pagine: 1084 - 1094
SICI:
0006-3363(1998)59:5<1084:AIFOTS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPRODUCTIVE-SYSTEM; THYROID-HORMONE; ANDROGEN RECEPTORS; EXCURRENT DUCTS; SPERM COUNTS; TESTIS; SPERMATOGENESIS; EXPRESSION; LEYDIG; INHIBIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Sharpe et al., "ABNORMALITIES IN FUNCTIONAL-DEVELOPMENT OF THE SERTOLI CELLS IN RATS TREATED NEONATALLY WITH DIETHYLSTILBESTROL - A POSSIBLE ROLE FOR ESTROGENS IN SERTOLI-CELL DEVELOPMENT", Biology of reproduction, 59(5), 1998, pp. 1084-1094

Abstract

Diethylstilbestrol (DES) was administered neonatally (Days 2-12; 10 mu g on alternate days) to rats, and developmental changes in Sertoli cell function were evaluated at 18, 25, and 35 days of age and comparedto those observed in rats administered a GnRH antagonist (GnRHa; Days2 and 5; 10 mg/kg) or a vehicle (controls). DES and GnRHa treatments resulted in similar reductions in both Sertoli cell numbers (40% for DES, 48% for GnRHa) and suppression of testicular growth at 18 and 25 days, though by 35 days the suppression was more pronounced (p < 0.001)in DES-treated animals. Plasma FSH levels were suppressed markedly at18 and 25 days, but not at 35 days, in GnRHa-treated rats,whereas in DES-treated rats the FSH levels were suppressed significantly only at 35 days. Both treatments suppressed plasma levels of inhibin B, thoughthis was more pronounced (p < 0.05) in DES- than in GnRHa-treated rats. In controls, Sertoli cell immunoexpression of inhibin a, sulfated glycoprotein-1 (SGP-1), and androgen receptor (AR) increased in intensity and changed to an adult, stage-dependent pattern by 25 days. In GnRHa-treated rats these changes were reduced in Intensity but were similar to those in controls at 35 days. In DES-treated rats, the increase in intensity and stage-dependent pattern of immunoexpression of inhibin alpha, SGP-1, and AR were virtually absent at 25 days but were present by 35 days. Germ cell volume per Sertoli cell was reduced in GnRHa-and DES-treated rats compared with controls at 18 and 25 days but wassignificantly greater (p < 0.001) in DES- than in GnRHa-treated rats at 35 days. The proportion of apoptotic to viable germ cells was increased (p < 0.01) in GnRHa- and DES-treated rats compared with controls at 18 and 25 days; but at 35 days, values in GnRHa-treated rats had declined to control values whereas those for DES-treated rats remained 10-fold elevated (p < 0.001). In adulthood, testis weight and daily sperm production were reduced by 43% and 44%, respectively, in GnRHa-treated rats, but spermatogenesis was grossly normal. Comparable changes were observed in similar to 25% of DES-treated rats, but the majority exhibited > 60% reduction in testis weight with many Sertoli cell-only tubules and very low daily sperm production. Taken together, these data are interpreted as providing evidence for direct modulation of Sertoli cell (maturational) development by DES.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:02:48