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Titolo:
CA2-INDUCED INHIBITION OF SODIUM-PUMP - EFFECTS ON ENERGETIC METABOLISM OF MOUSE DIAPHRAGM TISSUE()
Autore:
SULOVA Z; VYSKOCIL F; STANKOVICOVA T; BREIER A;
Indirizzi:
SLOVAK ACAD SCI,INST MOL PHYSIOL & GENET,PROT CHEM LAB,VLARSKA 5 SK-83334 BRATISLAVA SLOVAKIA SLOVAK ACAD SCI,INST MOL PHYSIOL & GENET,PROT CHEM LAB SK-83334 BRATISLAVA SLOVAKIA SLOVAK ACAD SCI,INST CHEM BRATISLAVA SLOVAKIA ACAD SCI CZECH REPUBL,INST PHYSIOL PRAGUE CZECH REPUBLIC COMENIUS UNIV,FAC PHARM BRATISLAVA SLOVAKIA
Titolo Testata:
General physiology and biophysics
fascicolo: 3, volume: 17, anno: 1998,
pagine: 271 - 283
SICI:
0231-5882(1998)17:3<271:CIOS-E>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
(NA+ + K+)-ATPASE; RED-BLOOD-CELLS; SKELETAL-MUSCLE; CALCIUM-INHIBITION; NEURONAL CELLS; RAT-HEART; MODULATION; HEMOLYSATE; CHANNELS; PARADOX;
Keywords:
SODIUM PUMP; INTRACELLULAR CALCIUM; GLUCOSE UPTAKE; RESPIRATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
Z. Sulova et al., "CA2-INDUCED INHIBITION OF SODIUM-PUMP - EFFECTS ON ENERGETIC METABOLISM OF MOUSE DIAPHRAGM TISSUE()", General physiology and biophysics, 17(3), 1998, pp. 271-283

Abstract

Tissues of mouse diaphragms were incubated in Liley solution containing 2, 4, 6 and 10 mmol/l calcium. When diaphragm tissue was incubated in 10 mmol/l calcium, an increase of intracellular calcium concentration from 314 +/- 28 to 637 +/- 26 nmol/l was estimated by fluorescent Ca2+ indicator Fura-2/AM. Moreover, incubation of the tissue in 10 mmol/l Ca2+ led to complete inhibition of electrogenic activity of the sodium pump, as measured by intracellular microelectrodes in a single muscle cell. This inhibition was fully reversible after 5 min washing with Liley solution containing 2 mmol/l CaCl2. The Ca2+-induced blocking effect on electrogenic activity of the sodium pump was accompanied by inhibition of glucose incorporation into the muscle tissue. Calcium atconcentrations of 6 and 10 mmol/l in bath medium significantly inhibited both CO2 production and O-2 consumption. A continual decrease of respiration (CO2/O-2) quotient was observed under increasing concentrations of calcium. Moreover, an exponential decrease of ATP tissue levels was observed at increasing concentrations of calcium in the bath medium. On the other hand, massive acceleration of anaerobic glycolysis induced by incubation of the tissue in a medium containing high calciumconcentration is improbable. This may be deduced from the fact that only about an 50% increase of lactate content in muscle tissue was observed when diaphragms were incubated for 30 min in medium containing calcium ions at 6 and 10 mmol/l as compared with the control tissue incubated for the same time in the medium containing 2 mmol/l CaCl2. In conclusion it could be stressed that increase of Ca2+ concentration in bath medium induced in diaphragm muscle tissue an elevation of intracellular Ca2+ concentration accompanied by a depression of sodium pump electrogenic activity and a depression of energy metabolism. These changes may be involved in pathology of muscle tissue during the Ca2+ overload.

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Documento generato il 06/04/20 alle ore 08:39:44