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Titolo:
THE POLYCYSTIC KIDNEY DISEASE-1 PROTEIN, POLYCYSTIN-1, BINDS AND ACTIVATES HETEROTRIMERIC G-PROTEINS IN-VITRO
Autore:
PARNELL SC; MAGENHEIMER BS; MASER RL; RANKIN CA; SMINE A; OKAMOTO T; CALVET JP;
Indirizzi:
UNIV KANSAS,MED CTR,DEPT BIOCHEM & MOL BIOL,3901 RAINBOW BLVD KANSAS CITY KS 66160 UNIV KANSAS,MED CTR,DEPT BIOCHEM & MOL BIOL KANSAS CITY KS 66160 CLEVELAND CLIN FDN,LERNER RES INST,DEPT NEUROSCI CLEVELAND OH 44195
Titolo Testata:
Biochemical and biophysical research communications (Print)
fascicolo: 2, volume: 251, anno: 1998,
pagine: 625 - 631
SICI:
0006-291X(1998)251:2<625:TPKDPP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUCINE-RICH REPEAT; SEQUENCE-ANALYSIS; PKD1; GENE; ENCODES; BRAIN; RECEPTOR; DOMAINS; MODEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S.C. Parnell et al., "THE POLYCYSTIC KIDNEY DISEASE-1 PROTEIN, POLYCYSTIN-1, BINDS AND ACTIVATES HETEROTRIMERIC G-PROTEINS IN-VITRO", Biochemical and biophysical research communications (Print), 251(2), 1998, pp. 625-631

Abstract

Analysis of the C-terminal cytosolic domain of human and mouse polycystin-1 has identified a number of conserved protein motifs, including a 20-amino-acid heterotrimeric G-protein activation sequence. A peptide specific for this sequence was synthesized and shown to activate purified bovine brain heterotrimeric Gi/Go in vitro. To test whether the C-terminal cytosolic domain of polycystin-1 stably binds G-proteins, GST-fusion constructs were used in pull-down and co-immunoprecipitationassays with purified bovine brain Gi/Go and rat brain lysates. This identified a 74-amino-acid minimal binding domain that includes the G-protein activation sequence. This region of polycystin-1, including theG-protein activation peptide and flanking amino acid sequences, is highly conserved in mouse, human, and puffer fish, lending further support to the functional importance of the minimal binding domain. These results suggest that polycystin-1 may function as a heterotrimeric G-protein coupled receptor, (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:50:34