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Titolo:
STRUCTURAL BASIS FOR THE INHIBITION OF FIREFLY LUCIFERASE BY A GENERAL ANESTHETIC
Autore:
FRANKS NP; JENKINS A; CONTI E; LIEB WR; BRICK P;
Indirizzi:
UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,BLACKETT LAB,BIOPHYS SECT,PRINCE CONSORT RD LONDON SW7 2BZ ENGLAND
Titolo Testata:
Biophysical journal
fascicolo: 5, volume: 75, anno: 1998,
pagine: 2205 - 2211
SICI:
0006-3495(1998)75:5<2205:SBFTIO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN; SITE; MECHANISMS; RECEPTORS; BINDING; ACT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
N.P. Franks et al., "STRUCTURAL BASIS FOR THE INHIBITION OF FIREFLY LUCIFERASE BY A GENERAL ANESTHETIC", Biophysical journal, 75(5), 1998, pp. 2205-2211

Abstract

The firefly luciferase enzyme from Photinus pyralis is probably the best-characterized model system for studying anesthetic-protein interactions. It binds a diverse range of general anesthetics over a large potency range, displays a sensitivity to anesthetics that is very similar to that found in animals, and has an anesthetic sensitivity that canbe modulated by one of its substrates (ATP). In this paper we describe the properties of bromoform acting as a general anesthetic (in Rana temporaria tadpoles) and as an inhibitor of the firefly luciferase enzyme at high and low ATP concentrations. In addition, we describe the crystal structure of the low-ATP form of the luciferase enzyme in the presence of bromoform at 2.2-Angstrom resolution. These results providea structural basis for understanding the anesthetic inhibition of theenzyme, as well as an explanation for the ATP modulation of its anesthetic sensitivity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:23:13