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Titolo:
REDUCTION OF THE DIAGNOSTIC WINDOW WITH A NEW COMBINED P24 ANTIGEN AND HUMAN-IMMUNODEFICIENCY-VIRUS ANTIBODY SCREENING ASSAY
Autore:
GURTLER L; MUHLBACHER A; MICHL U; HOFMANN H; PAGGI GG; BOSSI V; THORSTENSSON R; VILLAESCUSA RG; EIRAS A; HERNANDEZ JM; MELCHIOR W; DONIE F; WEBER B;
Indirizzi:
UNIV KLINIKEN FRANKFURT,INST MED VIROL FRANKFURT GERMANY UNIV KLINIKEN FRANKFURT,INST MED VIROL FRANKFURT GERMANY BOEHRINGER MANNHEIM GMBH DENZBERG GERMANY MAX VON PETTENKOFER INST HYG & MED MICROBIOL MUNICH GERMANY BLUTZENT LANDESKRANKENANSTALTEN SALZBURG SALZBURG AUSTRIA UNIV VIENNA,INST VIROL A-1095 VIENNA AUSTRIA SSN REG PIEMONTE USSL,PRESIDIO OSPED AMAEDEO SAVOIA,VIROL LAB TURIN ITALY SWEDISH INST INFECT DIS CONTROL STOCKHOLM SWEDEN CTR REG TRANSFUS GALICIA SANTIAGO COMPOSTE SPAIN HOSP GEN VALLE HEBRON,ICS,BANCO SANGRE BARCELONA SPAIN CTR LANGWIES,LABS REUNIS KUTTER LIENERS HASTERT L-6131 JUNGLINSTER LUXEMBOURG
Titolo Testata:
Journal of virological methods
fascicolo: 1, volume: 75, anno: 1998,
pagine: 27 - 38
SICI:
0166-0934(1998)75:1<27:ROTDWW>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-DONORS; HIV-1; SUBTYPE; INFECTION;
Keywords:
SEROCONVERSION; COMBINED ANTIGEN AND ANTIBODY DETECTION; GENETIC VARIABILITY; HIV-1 SUBTYPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
L. Gurtler et al., "REDUCTION OF THE DIAGNOSTIC WINDOW WITH A NEW COMBINED P24 ANTIGEN AND HUMAN-IMMUNODEFICIENCY-VIRUS ANTIBODY SCREENING ASSAY", Journal of virological methods, 75(1), 1998, pp. 27-38

Abstract

In order to reduce the window phase between time of human immunodeficiency virus (HIV) infection and laboratory diagnosis, new fourth generation screening assays which permit a simultaneous detection of HIV antigen and antibody have been developed. In a multicenter study, a new automated fourth generation assay, Enzymun-Test(R) HIV Combi (Boehringer Mannheim GmbH) was compared to third generation assay, p24 antigen tests and Western blot. A total of 37 seroconversion panels, samples of the early infection (n = 42), HIV-1 antibody positive sera, including subtypes A-E, and O (n = 1118), HIV-2 positive samples (n = 252) andcell culture supernatants infected with different HIV-1 subtypes and HIV-2 (n = 50), blood donors (n = 6649), hospitalized patients (n = 475), HIV neg. sera with indeterminate Western blot (n = 32), potentially cross reactive serum samples (n = 435) and HIV negative specimens from Cameroon (n = 68) were tested. A total of 16 of 29 seroconversions were detected on average 8.5 days earlier with Enzymun-Test(R) HIV Combi than HIV-1/HIV-2 3rd generation EIA (Abbott Laboratories). Overall,in the 29 panels investigated comparatively with the two assays, the mean time delay between Enzymun-Test(R) HIV Combi and HIV-1/HIV-2 3rd generation EIA was 4.7 days. HIV antigen was detected in three out of 35 seroconversions one bleed earlier with HIV-1 Ag Monoclonal than with Enzymun-Test(R) HIV Combi. Enzymun-Test(R) HIV Combi showed a sensitivity of 100% for HIV antibody detection for HIV-1 group hi and O and HIV-2 positive specimens. While p24 antigen of different HIV-I subtypes was detected with Enzymun-Test(R) HIV Combi in all the 49 cell culture supernatants, HIV Ag was not detected in an HIV-2 virus lysate. A total of 66 false positive results out of 7659 HIV negative samples were obtained with the Enzymun-Test(R) HIV Combi. The specificity for unselected blood donors was 99.6%. The Enzymun-Test(R) HIV Combi permits an earlier diagnosis of HIV infection than third generation assays through the detection of p24 antigen, which may be present in serum samples from individuals with recent HIV infection prior to seroconversion and it shows an excellent sensitivity for antibodies to all known HIV-I subtypes and HIV-2. The specificity in blood donors and hospitalizedpatients is comparable to that of other assays. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 14:40:45