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Titolo:
A COLORIMETRIC REVERSE-TRANSCRIPTASE ASSAY OPTIMIZED FOR MOLONEY MURINE LEUKEMIA-VIRUS, AND ITS USE FOR CHARACTERIZATION OF REVERSE TRANSCRIPTASES OF UNKNOWN IDENTITY
Autore:
MALMSTEN A; EKSTRAND DHL; AKERBLOM L; GRONOWITZ JS; KALLANDER CFR; BENDINELLI M; MATTEUCCI D;
Indirizzi:
UNIV LEEDS,SCH BIOCHEM & MOL BIOL LEEDS LS2 9JT W YORKSHIRE ENGLAND UPPSALA UNIV,BMC,DEPT GENET & PATHOL S-75123 UPPSALA SWEDEN NATL VET INST,DEPT VIROL S-75007 UPPSALA SWEDEN CAVIDI TECH AB S-75183 UPPSALA SWEDEN UNIV PISA,DEPT BIOMED,RETROVIRUS CTR I-56127 PISA ITALY UNIV PISA,DEPT BIOMED,VIROL SECT I-56127 PISA ITALY
Titolo Testata:
Journal of virological methods
fascicolo: 1, volume: 75, anno: 1998,
pagine: 9 - 20
SICI:
0166-0934(1998)75:1<9:ACRAOF>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
FELINE IMMUNODEFICIENCY VIRUS; ACTIVITY-BLOCKING ANTIBODY; DEPENDENT DNA POLYMERASE; ROUS-SARCOMA VIRUS; GENE-TRANSFER; RETROVIRAL VECTORS; MAMMALIAN-CELLS; HIGH-TITER; INFECTION; TYPE-1;
Keywords:
RETROVIRUS; MOLONEY MURINE LEUKEMIA VIRUS; FELINE LEUKEMIA VIRUS; REVERSE TRANSCRIPTASE (RT); RT ACTIVITY BLOCKING ANTIBODY; ECOTROPIC RECOMBINANT VIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
A. Malmsten et al., "A COLORIMETRIC REVERSE-TRANSCRIPTASE ASSAY OPTIMIZED FOR MOLONEY MURINE LEUKEMIA-VIRUS, AND ITS USE FOR CHARACTERIZATION OF REVERSE TRANSCRIPTASES OF UNKNOWN IDENTITY", Journal of virological methods, 75(1), 1998, pp. 9-20

Abstract

A non-radioactive reverse transcriptase (RT) assay, reported as useful for lentivirus RTs, was optimized for the measurement of Moloney murine leukemia virus (MMuLV) RT. The optimized assay could detect 0.3 muU of MMuLV RT. The specificities of the MMuLV and lenti RT assays were demonstrated using the RTs of human immunodeficiency virus type 1, simian immunodeficiency virus, feline immunodeficiency virus (FIV). visna virus, human T-cell lymphotropic virus type 1, MMuLV and feline leukemia virus (FeLV). An RT activity blocking antibody (RTb-ab) assay was standardized for Mn2+ dependent MuLV-related RTs. The assay was usedto demonstrate the distinct antigenic properties of RTs from mammalian MuLV-related retroviruses and lentiviruses. Cross-reactivity betweenMMuLV RTb-ab and FeLV RT but not between MMuLV RTb-ab and e.g. FIV RTwas demonstrated. An RT activity found in the murine myeloma cell line SP2/0 was found to have similar assay preferences as MMuLV RT, and the MMuLV-RT hyperimmune sera reacted strongly against this RT, indicating the RT to be of MuLV-related etiology. The use of the RT and RTb-ab assays for detection and characterization of RTs of known or unknownidentity is discussed. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 16:49:58