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Titolo:
PROMOTER CHARACTERIZATION AND STRUCTURE OF THE GENE ENCODING MOUSE LYSOSOMAL ALPHA-D-MANNOSIDASE
Autore:
STINCHI S; ORLACCHIO A; COSTANZI E; STIRLING JL; MENGHINI AR; ORLACCHIO A; BECCARI T;
Indirizzi:
UNIV PERUGIA,DIPARTIMENTO BIOL CELLULARE & MOL,VIA GIOCHETTO I-06126 PERUGIA ITALY UNIV PERUGIA,DIPARTIMENTO BIOL CELLULARE & MOL I-06126 PERUGIA ITALY UNIV LONDON KINGS COLL,DIV LIFE SCI LONDON W8 7AH ENGLAND
Titolo Testata:
Mammalian genome
fascicolo: 11, volume: 9, anno: 1998,
pagine: 869 - 873
SICI:
0938-8990(1998)9:11<869:PCASOT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASSIGNMENT; EXPRESSION; IDENTIFICATION; PURIFICATION; CLONING; CDNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
S. Stinchi et al., "PROMOTER CHARACTERIZATION AND STRUCTURE OF THE GENE ENCODING MOUSE LYSOSOMAL ALPHA-D-MANNOSIDASE", Mammalian genome, 9(11), 1998, pp. 869-873

Abstract

Mouse lysosomal alpha-D-mannosidase (EC 3.2.1.24) is an enzyme involved in the catabolism of N-linked glycoproteins. The gene is differentially expressed in mouse tissues, and the highest level of mRNA is found in the epididymis. The expression of mannosidase in the epididymis may be hormonally regulated, since its activity increases with age. To understand the factors affecting the expression of mouse mannosidase, we isolated and characterized the promoter and determined the exon-intron structure. The gene is about 15 kb, consists of 24 exons, and the 5' flanking region contains GC-rich regions, TATA boxes, CAAT boxes, and putative binding sites for the transcription factors Spl, AP2, and PEA3. PEA3 factor may participate in the transcriptional control of mannosidase expression in the mouse epididymis. In fact, it has been demonstrated that the PEA3 motif is spatially and temporally expressed within the mouse epididymis, and its accumulation is controlled by androgens and testicular factors. A 1279-bp fragment from the initiation codon had the strongest promoter activity, and three different transcription start sites were identified at positions -131, -149, and -174.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:34:03