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Titolo:
GENE DISCOVERY THROUGH EXPRESSED SEQUENCE TAG SEQUENCING IN TRYPANOSOMA-CRUZI
Autore:
VERDUN RE; DIPAOLO N; URMENYI TP; RONDINELLI E; FRASCH ACC; SANCHEZ DO;
Indirizzi:
UNIV NACL GEN SAN MARTIN,INTI ED 24,INST INVEST BIOTECNOL RA-1650 SANMARTIN BUENOS AIRES ARGENTINA UNIV NACL GEN SAN MARTIN,INTI ED 24,INST INVEST BIOTECNOL RA-1650 SANMARTIN BUENOS AIRES ARGENTINA FED UNIV RIO DE JANEIRO,INST BIOFIS CARLOS CHAGAS FILHO RIO JANEIRO BRAZIL
Titolo Testata:
Infection and immunity (Print)
fascicolo: 11, volume: 66, anno: 1998,
pagine: 5393 - 5398
SICI:
0019-9567(1998)66:11<5393:GDTEST>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENOME PROJECT; SURFACE PROTEIN; CDNA LIBRARY; IDENTIFICATION; LEISHMANIA; FAMILY; MEMBER; PROMASTIGOTES; SUPERFAMILY; MACROPHAGES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
R.E. Verdun et al., "GENE DISCOVERY THROUGH EXPRESSED SEQUENCE TAG SEQUENCING IN TRYPANOSOMA-CRUZI", Infection and immunity (Print), 66(11), 1998, pp. 5393-5398

Abstract

Analysis of expressed sequence tags (ESTs) constitutes a useful approach for gene identification that, in the case of human pathogens, might result in the identification of new targets for chemotherapy and vaccine development. As part of the Trypanosoma cruzi genome project, we have partially sequenced the 5' ends of 1,949 clones to generate ESTs,The clones were randomly selected from a normalized CL Brener epimastigote cDNA library. A total of 14.6% of the clones were homologous to previously identified T. cruzi genes, while 18.4% had significant matches to genes from other organisms in the database. A total of 67% of the ESTs had no matches in the database, and thus, some of them might be T. cruzi-specific genes. Functional groups of those sequences with matches in the database were constructed according to their putative biological functions, The two largest categories were protein synthesis (23.3%) and cell surface molecules (10.8%). The information reported in this paper should be useful for researchers in the field to analyze genes and proteins of their own interest.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:12:37