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Titolo:
EXPRESSION OF THE TPR PROTEASE GENE OF PORPHYROMONAS-GINGIVALIS IS REGULATED BY PEPTIDE NUTRIENTS
Autore:
LU BQ; MCBRIDE BC;
Indirizzi:
UNIV BRITISH COLUMBIA,DEPT MICROBIOL & IMMUNOL,6328 MEM RD VANCOUVER BC V6T 1Z2 CANADA UNIV BRITISH COLUMBIA,DEPT MICROBIOL & IMMUNOL VANCOUVER BC V6T 1Z3 CANADA UNIV BRITISH COLUMBIA,DEPT ORAL BIOL & MED SCI VANCOUVER BC V6T 1Z3 CANADA
Titolo Testata:
Infection and immunity (Print)
fascicolo: 11, volume: 66, anno: 1998,
pagine: 5147 - 5156
SICI:
0019-9567(1998)66:11<5147:EOTTPG>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTEROIDES-GINGIVALIS; ESCHERICHIA-COLI; NUCLEOTIDE-SEQUENCE; HEMAGGLUTININ GENE; ENZYME-ACTIVITY; HEMIN-BINDING; CLONING; W50; PROTEINASE; ELEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
B.Q. Lu e B.C. Mcbride, "EXPRESSION OF THE TPR PROTEASE GENE OF PORPHYROMONAS-GINGIVALIS IS REGULATED BY PEPTIDE NUTRIENTS", Infection and immunity (Print), 66(11), 1998, pp. 5147-5156

Abstract

The Tpr protease of Porphyromonas gingivalis W83 is a membrane-associated enzyme capable of hydrolyzing chromogenic substrates for trypsin and bacterial collagenases. A previous study by us indicated that Tpr expression was increased under conditions of nutrient limitation. In the present study, we further characterized expression of the tpr gene using a tpr::lacZ reporter gene construct under a range of nutrient conditions. In P. gingivalis, transcription of tpr was initiated 215 bp upstream of the coding region and regulation of tpr expression was at the level of transcription. Deletion mutations in the tpr upstream region identified the promoter region immediately upstream of the transcription start site, determined by primer extension analysis. Three identical 17-bp direct repeats identified,within the 5' end of tpr mRNA were involved in tpr regulation. In an Escherichia call background, tpr transcription was initiated after an AT-rich region upstream of tpr but not at the P. gingivalis start site. Tpr expression in P. gingivaliswas suppressed by the addition of peptide and protein nutrients to a peptide-limited growth medium but was only slightly affected by addition of free amino acids. Low-molecular-weight fractions of brain heart infusion rich in phenylalanine, proline, and alanine had the greatest inhibitory effects on expression of the tpr::lacZ construct. Addition of the dipeptide phenylalanyl-phenylalanine to the growth medium resulted in a 10-fold decrease in tpr expression. This suggests that specific phenylalanine-containing peptides are a major factor controlling Tpr expression. Neither hemin starvation, heat shock, nor pH change had significant effects on Tpr expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:33:23