Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
KERATINOCYTE GROWTH-FACTOR INCREASES TRANSALVEOLAR SODIUM-REABSORPTION IN NORMAL AND INJURED RAT LUNGS
Autore:
GUERY RPH; MASON CM; DOBARD EP; BEAUCAIRE G; SUMMER WR; NELSON S;
Indirizzi:
CTR HOSP TOURCOING,SERV REANIM MED & MALAD INFECT,135 RUE PDT COTY F-59208 TOURCOING FRANCE LOUISIANA STATE UNIV,MED CTR,DEPT MED NEW ORLEANS LA 70112
Titolo Testata:
American journal of respiratory and critical care medicine
fascicolo: 5, volume: 155, anno: 1997,
pagine: 1777 - 1784
SICI:
1073-449X(1997)155:5<1777:KGITS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED PULMONARY-EDEMA; EPITHELIAL-CELLS; II CELLS; VASCULAR-PERMEABILITY; ALVEOLAR EPITHELIUM; TRANSPORT; EXPRESSION; MONOLAYERS; CLEARANCE; ANTU;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
R.P.H. Guery et al., "KERATINOCYTE GROWTH-FACTOR INCREASES TRANSALVEOLAR SODIUM-REABSORPTION IN NORMAL AND INJURED RAT LUNGS", American journal of respiratory and critical care medicine, 155(5), 1997, pp. 1777-1784

Abstract

Keratinocyte growth factor (KGF) prevents alpha-naphthylthiourea (ANTU)-induced permeability edema ex vivo. To explore the mechanisms in this involved effect, we administered KGF (5 mg/kg, intratracheally) 48 h prior to ANTU (50 mg/kg, intraperitoneally). Several groups were studied: phosphate-buffered saline/dimethylsuIfoxide (PBS/DMSO) (vehicles), PBS/ANTU, and KGF/ANTU. At 90 min after ANTU injection the lungs were removed, ventilated, and perfused ex vivo for 180 min. Quantification of fluorescein isothiocyanate (FITC)-labeled dextran in bronchoalveolar lavage fluid (BALF) was used to assess alveolar capillary barrierpermeability. KGF attenuated ANTU-induced edema and blockade of sodium transport, with ouabain (10(-3) M) or amiloride (10(-4) M) added ex vivo reversed this effect, FITC-dextran was increased in the PBS/ANTU group as compared with the PBS/DMSO group, indicating permeability edema. In the KGF/ANTU group, there was concentration of BALF FITC-dextran, consistent with permeability edema and increased alveolar fluid export. Albumin space measurements showed similar increases in permeability in the PBS/ANTU and KGF/ANTU groups. Extravascular lung water (measured with radiolabeled erythrocytes) was decreased in the KGF/ANTU group. Following KGF pretreatment, uninjured lungs exported more intratracheal PBS than normal lungs following terbutaline stimulation ex vivo. In conclusion, KGF, through type II alveolar pneumocyte hyperplasia with increased sodium-potassium-adenosine triphosphatase (Na,K-ATPase) activity, attenuated ANTU-induced edema formation by potentiating alveolar fluid clearance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 04:42:28