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Titolo:
ALL-TRANS-RETINOIC ACID COMPROMISES DESMOSOME EXPRESSION IN HUMAN EPIDERMIS
Autore:
HUMPHRIES JD; PARRY EJ; WATSON REB; GARROD DR; GRIFFITHS CEM;
Indirizzi:
UNIV MANCHESTER,HOPE HOSP,DEPT MED,DERMATOL SECT SALFORD M6 8HD LANCSENGLAND UNIV MANCHESTER,HOPE HOSP,DEPT MED,DERMATOL SECT SALFORD M6 8HD LANCSENGLAND UNIV MANCHESTER,SCH BIOL SCI,EPITHELIAL MORPHOGENESIS RES GRP MANCHESTER M13 9PL LANCS ENGLAND
Titolo Testata:
British journal of dermatology
fascicolo: 4, volume: 139, anno: 1998,
pagine: 577 - 584
SICI:
0007-0963(1998)139:4<577:AACDEI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SKIN; DIFFERENTIATION; CADHERINS; FRAGILITY; ISOFORMS; PATTERNS; GENE; IDENTIFICATION; PEMPHIGUS; EPITHELIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J.D. Humphries et al., "ALL-TRANS-RETINOIC ACID COMPROMISES DESMOSOME EXPRESSION IN HUMAN EPIDERMIS", British journal of dermatology, 139(4), 1998, pp. 577-584

Abstract

An undesirable side-effect of retinoid treatment is skin fragility, As desmosomes are important in maintaining the cohesion of epidermal keratinocytes, we investigated whether all-trans-retinoic acid (RA) compromises desmosome expression in human epidermis, thereby predisposing skin to fragility, Solutions containing 0.025% RA, 5% sodium dodecyl sulphate (SDS) as an irritant control, or vehicle alone were applied tothree sites on the buttocks of normal volunteers (n = 9). Treated sites were occluded for 4 days, and biopsies taken under local anaesthesia. Cryostat sections were stained with a panel of antibodies to desmosomal proteins and visualized by immunofluorescence microscopy. Stainedsections were randomized and assessed for intensity of staining. The epidermal thickness of each treated site was quantified by image analysis. Western blots of epidermal desmocollins were quantified by densitometry. RA and SDS treatments significantly, but equivalently, increased epidermal thickness compared with vehicle, Immunohistochemically, both RA and SDS were shown to reduce epidermal staining for desmoplakin, desmoglein 1, plakophilin 1 and desmocollin 3 equally compared with vehicle-treated skin (P < 0.001). RA produced a greater reduction in desmocollin 1 staining compared with SDS (P < 0.001). Similar reductions in desmocollins were found by Western blot analysis. Reduced desmocollin expression may indicate compromised desmosomal adhesion, leading to the skin fragility that results from retinoid treatment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:30:52