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Titolo:
PREPARATION AND CHARACTERIZATION OF ENTERIC MICROSPHERES CONTAINING BOVINE INSULIN BY A WOW EMULSION SOLVENT EVAPORATION METHOD
Autore:
NAGAREYA N; UCHIDA T; MATSUYAMA K;
Indirizzi:
MUKOGAWA WOMENS UNIV,FAC PHARMACEUT SCI,11-68 KOSHIEN 9 BANCHO NISHINOMIYA HYOGO 6638179 JAPAN MUKOGAWA WOMENS UNIV,FAC PHARMACEUT SCI NISHINOMIYA HYOGO 6638179 JAPAN
Titolo Testata:
Chemical and Pharmaceutical Bulletin
fascicolo: 10, volume: 46, anno: 1998,
pagine: 1613 - 1617
SICI:
0009-2363(1998)46:10<1613:PACOEM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYLACTIC ACID MICROSPHERES; SIZE DISTRIBUTION; MICROCAPSULES; MICROENCAPSULATION;
Keywords:
MICROSPHERE; ENTERIC POLYMER; BOVINE INSULIN; HYDROXYPROPYLMETHYLCELLULOSE ACETATE SUCCINATE; LAURIC ACID; PLASMA GLUCOSE LEVEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
N. Nagareya et al., "PREPARATION AND CHARACTERIZATION OF ENTERIC MICROSPHERES CONTAINING BOVINE INSULIN BY A WOW EMULSION SOLVENT EVAPORATION METHOD", Chemical and Pharmaceutical Bulletin, 46(10), 1998, pp. 1613-1617

Abstract

The objective of this study was to produce enteric microspheres containing bovine insulin as a model drug using a water-in-oil-in-water (w/o/v) emulsion solvent evaporation method, and the preparative conditions were optimized. When hydroxypropylmethylcellulose acetate succinate(AS-HG type; high content of succinyl group) was employed as an enteric wall material, optimized microspheres showed almost 90% of the loading efficiency of insulin and 30.8 mu m of mean volume diameter. The mixture of methylene chloride and acetone (4:1) as an oleaginous phase,400 mu l of bovine insulin solution (dissolved in 30% of acetic acid)as an internal aqueous phase, and 1.0% of polyvinylalcohol dissolved in pH 3.0 citrate buffer as an external aqueous phase, were employed in the experiment. In relation to other enteric cellulose derivatives (AS-MG type, AS-LG type; medium and low content of succinyl group, respectively), the microencapsulation using a simultaneous preparation method also resulted in quite high loading efficiencies, whereas the choice of poly(methyl methacrylate) as a wall material caused aggregation or flocculation in the preparative process of every batch. The AS-MG microspheres showed very fast release profile in pH 6.8 buffer, but no released fraction was observed in pH 1.2 buffer. This phenomenon suggested enteric characteristics of prepared microspheres. Finally AS-MG microspheres containing 4% lauric acid and 9% insulin were prepared, suspended in 0.1% of carboxymethyl cellulose solution, and administered to the rat rectum (corresponding to 50 I.U./kg insulin). The plasma glucose level reached minimum level at 0.5 h after administration then gradually rose to normal.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 10:48:11