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Titolo:
INSULIN-LIKE GROWTH-FACTOR-I EFFECT ON CHICKEN HEPATOMA-CELLS (LMH) IS INHIBITED BY ENDOGENOUS IGF-BINDING PROTEINS
Autore:
DUCLOS MJ; CHEVALIER B; UPTON Z; SIMON J;
Indirizzi:
INRA,RECH AVICOLES STN F-37380 NOUZILLY FRANCE COOPERAT RES CTR TISSUE GROWTH & REPAIR ADELAIDE SA 5000 AUSTRALIA
Titolo Testata:
Growth hormone & IGF research
fascicolo: 2, volume: 8, anno: 1998,
pagine: 97 - 103
SICI:
1096-6374(1998)8:2<97:IGEOCH>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; RECEPTOR; LINE; MEMBRANES; SERUM; LIVER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
M.J. Duclos et al., "INSULIN-LIKE GROWTH-FACTOR-I EFFECT ON CHICKEN HEPATOMA-CELLS (LMH) IS INHIBITED BY ENDOGENOUS IGF-BINDING PROTEINS", Growth hormone & IGF research, 8(2), 1998, pp. 97-103

Abstract

LMH chicken hepatoma cells show type 1 IGF receptors and a 28 kDa IGF-binding protein (IGFBP) on their membranes. They also secrete large amounts of the 28 kDa IGFBP. Following overnight incubation in serum-free medium, human IGF-I was markedly less effective than insulin in stimulating amino acid (AIB) uptake. Chicken and human IGF-I were equipotent, consistent with their equipotency in inhibiting [I-125]IGF-I binding to wheat germ agglutinin-purified IGF receptors or membrane solubilized IGFBP. When cells were supplied with fresh medium, cell-associated IGFBP were unaffected, but the level of soluble IGFBP was largely reduced. This potentiated the effect of IGF-I on AIB uptake. The effectof chicken Long-[Arg(3)]-IGF-I, which exhibited low affinity for the IGFBP, was unchanged. In fresh or conditioned medium, this analog was more potent than IGF-I, suggesting that both soluble and membrane-bound 28 kDa IGFBP inhibited the effect of IGF-I. (C) 1998 Churchill Livingstone

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:53:05