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Titolo:
SER-262 IN HUMAN RECOMBINANT TAU-PROTEIN IS A MARKEDLY MORE FAVORABLESITE FOR PHOSPHORYLATION BY CAMKII THAN PKA OR PHK
Autore:
SIRONI JJ; YEN SH; GONDAL JA; WU QL; GRUNDKEIQBAL I; IQBAL K;
Indirizzi:
NEW YORK STATE INST BASIC RES DEV DISABIL,1050 FOREST HILL RD STATEN ISL NY 10314 NEW YORK STATE INST BASIC RES DEV DISABIL STATEN ISL NY 10314 MAYO CLIN JACKSONVILLE FL 32224
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 436, anno: 1998,
pagine: 471 - 475
SICI:
0014-5793(1998)436:3<471:SIHRTI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PAIRED HELICAL FILAMENTS; MICROTUBULE-ASSOCIATED PROTEINS; KINASE-II; FETAL TAU; DIFFERENTIAL PHOSPHORYLATION; ALZHEIMERS-DISEASE; SER(262); ISOFORMS; BRAIN; IDENTIFICATION;
Keywords:
CALCIUM CALMODULIN KINASE II; PROTEIN KINASE A; PHOSPHORYLASE KINASE; SER-262; TAU PHOSPHORYLATION; ALZHEIMERS DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
J.J. Sironi et al., "SER-262 IN HUMAN RECOMBINANT TAU-PROTEIN IS A MARKEDLY MORE FAVORABLESITE FOR PHOSPHORYLATION BY CAMKII THAN PKA OR PHK", FEBS letters, 436(3), 1998, pp. 471-475

Abstract

Several kinases have been shown to phosphorylate tau protein at Ser-262, an important site involved in the regulation of the binding of tauto microtubules, In this study we compared the phosphorylation of tauat Ser-262 by CaMKII, PhK and PKA in vitro as determined by radioimmunoblots developed by the monoclonal antibody 12E8 which recognizes P-Ser-262 and P-Ser-356; and Ab-262, a polyclonal antibody which is specific to unphosphorylated Ser-262 in tau. We found that the phosphorylation at Ser-262 was several times more effective by CaMKII than PKA or PhK. Employing rat brain extract as a source of all brain kinases and KN-62, a specific inhibitor of CaMKII, we found that CaMKII accounts for similar to 45% of phosphorylation at Ser-262. Furthermore, in rat brain slices kept metabolically active in oxygenated artificial CSF, phosphorylation of tau at Ser-262 was (i) increased up to 120% in time presence of bradykinin, a CaMKII activator, and (ii) inhibited by similar to 35% in the presence of KN-62, Thus, CaMKII is a major tau Ser-262 kinase in mammalian brain, (C) 1998 Federation of European Biochemical Societies.

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Documento generato il 21/09/20 alle ore 17:20:04