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Titolo:
EVIDENCE FOR AN IMPORTANT INTERACTION BETWEEN A COMPLEMENT-DERIVED CD21 LIGAND ON FOLLICULAR DENDRITIC CELLS AND CD21 ON B-CELLS IN THE INITIATION OF IGG RESPONSES
Autore:
QIN DH; WU JH; CARROLL MC; BURTON GF; SZAKAL AK; TEW JG;
Indirizzi:
VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT MICROBIOL IMMUNOL,DIV IMMUNOBIOL,POB 980678 RICHMOND VA 23298 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT MICROBIOL IMMUNOL,DIV IMMUNOBIOL RICHMOND VA 23298 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT ANAT,DIV IMMUNOBIOLRICHMOND VA 23298 HARVARD UNIV,SCH MED,DEPT PATHOL BOSTON MA 02115
Titolo Testata:
The Journal of immunology (1950)
fascicolo: 9, volume: 161, anno: 1998,
pagine: 4549 - 4554
SICI:
0022-1767(1998)161:9<4549:EFAIIB>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SECONDARY ANTIBODY-RESPONSE; HUMORAL IMMUNE-RESPONSE; T-DEPENDENT ANTIGEN; GERMINAL CENTER; ACQUIRED-IMMUNITY; LYMPHOCYTES-B; LYMPH-NODES; INVIVO; APOPTOSIS; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
D.H. Qin et al., "EVIDENCE FOR AN IMPORTANT INTERACTION BETWEEN A COMPLEMENT-DERIVED CD21 LIGAND ON FOLLICULAR DENDRITIC CELLS AND CD21 ON B-CELLS IN THE INITIATION OF IGG RESPONSES", The Journal of immunology (1950), 161(9), 1998, pp. 4549-4554

Abstract

The addition of Ags to mononuclear leukocyte cultures typically elicits modest Ab responses, implying that cosignals beyond those provided by T cells and macrophages may be needed. Recently, we reported that Ab responses could be dramatically enhanced (10-1000-fold) by the addition of follicular dendritic cells (FDC), suggesting that FDC may provide an important costimulatory signal. This result prompted a study of molecules involved in FDC-mediated enhancement of Ab responses stimulated by specific Ag with memory T and B cells or nonspecifically by theaddition of LPS, In this study, we report evidence supporting the concept that FDC bear a ligand that engages complement receptor II (CR2 or CD21) on B cells and provides a critical cosignal for both Ag-specific and polyclonal responses. A blockade of the CR2 ligand on FDC by the use of soluble CR2 or a blockade of CR2 on B cells by use of CR2 knockout mice (or B cells with CR2 blocked) reduced Ab responses from themu g/ml to the ng/ml range (10-1000-fold reductions). FDC from C3 knockout mice, which cannot generate the CR2-binding fragments (iC3b, C3d, and C3dg), were unable to provide costimulatory activity, suggestingthe CR2 ligand on FDC consists of C3 fragments, FDC trap complement-activating Ag-Ab complexes, and it appears that FDC present B cells with both specific Ag to engage B cell receptors and a CR2 ligand to engage B cell-CR2, In short, optimal induction of specific Ab responses appears to require the combination of specific Ag and costimulatory molecules from both T cells and FDC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 11:41:45