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Titolo:
CHROMOSOMAL IN-VITRO RADIOSENSITIVITY OF LYMPHOCYTES IN RADIOTHERAPY PATIENTS AND AT-HOMOZYGOTES
Autore:
DUNST J; NEUBAUER S; BECKER A; GEBHART E;
Indirizzi:
UNIV HALLE WITTENBERG,KLIN STRAHLENTHERAPIE,DEPT RADIOONCOL D-06097 HALLE GERMANY UNIV ERLANGEN NURNBERG,DEPT RADIOONCOL D-8520 ERLANGEN GERMANY UNIV ERLANGEN NURNBERG,DEPT HUMAN GENET D-8520 ERLANGEN GERMANY
Titolo Testata:
Strahlentherapie und Onkologie
fascicolo: 10, volume: 174, anno: 1998,
pagine: 510 - 516
SICI:
0179-7158(1998)174:10<510:CIROLI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE INSITU HYBRIDIZATION; ATAXIA-TELANGIECTASIA; SKIN FIBROBLASTS; BREAST-CANCER; INTRINSIC RADIOSENSITIVITY; INVITRO RADIOSENSITIVITY; SUBCUTANEOUS FIBROSIS; SITU HYBRIDIZATION; CLINICAL-RESPONSES; RADIATION-THERAPY;
Keywords:
RADIOTHERAPY; RADIOSENSITIVITY; NORMAL TISSUE REACTION; FISH; ATAXIA TELANGIECTASIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
J. Dunst et al., "CHROMOSOMAL IN-VITRO RADIOSENSITIVITY OF LYMPHOCYTES IN RADIOTHERAPY PATIENTS AND AT-HOMOZYGOTES", Strahlentherapie und Onkologie, 174(10), 1998, pp. 510-516

Abstract

Background: We investigated the in-vitro radiosensitivity of peripheral blood lymphocytes with a special FISH/CISS-technique. Patients and Methods: From October 1993 through April 1996, a total number of 52 cancer patients was enrolled in the study. The tumor sites in these patients were: breast (n = 41),lung (n = 4), head and neck (n = 3) as wellas prostate, bladder, rectal cancer and Hodgkin's disease (each n = 1). Twenty-six of them were examined prior to planned radiotherapy (prospective group) and 26 after radiotherapy (retrospective group). Threeadditional individuals (without cancer or radiotherapy) with proven ataxia telangiectasia (Louis-Bar syndrome, AT-homozygotes) were also investigated and their blood samples served as positive control for radiosensitivity. The clinical radiation response of normal tissue in radiotherapy patients was scored according to the WHO grading system for acute and according to the RTOG grading system for late effects. For toestimate the intrinsic radiosensitivity, blood samples were taken andirradiated in vitro with 0 (control) or 0.7 or 2 Gy with a 6 MV-linear accelerator, standard 48-hour lymphocyte cultures were prepared, chromosomes #1, #2 and #4 were simultaneously labeled with a FISH/CISS-technique and 200 to 1000 metaphase spreads were scored for chromosomal aberrations. The radiation sensitivity of lymphocytes was expressed asthe number of radiation-induced chromosomal breaks per mitosis after 0.7 Gy or 2 Gy corrected for the 0-Gy control value. Results: The frequency of chromosomal breaks/mitosis in the unirradiated control lymphocytes was 0.020 +/- 0.015 in prospective patients who had not yet received radiotherapy. It was significantly higher in retrospective patients (0.264 +/- 0.164 breaks/mitosis) as a result of the previous radiation exposure. The 3 AT-homozygotes showed also an increased number of spontaneous chromosomal breaks (0.084 +/- 0.016 breaks/mitosis), probably resulting from the chromosomal instability in this disease. This figure, however, was significantly lower than in retrospective patients. - The number of radiation-induced breaks after in-vitro irradiation was comparable in lymphocytes of patients who showed no normal tissue reaction (n = 11) as compared to those with mild to moderate radiationreaction (n = 32, acute reactions Grade 1 to 2, late reactions Grade 0 to 2). In 9 patients with unexpected severe plus late Grade 3 to 4 reactions, however, a significantly higher number of radiation-induced chromosomal breaks was measured; the highest number was observed in a patient with a radiation myelitis. The 3 AT-homozygotes showed, as expected, an extreme radiosensitivity of their lymphocytes. The number ofbreaks/mitosis after 0.7 Gy in vitro irradiation of lymphocytes was 0.103 +/- 0.059 in patients with no normal tissue radiation reaction (n= 11), 0.122 +/- 0.146 in the group with mild to moderate radiation reactions Grade 1 to 2 (n = 32), 0.359 + 0.226 in patients with unexpected Grade 3 to 4 normal tissue reactions (n = 9) and 0.550 +/- 0.243 in the 3 AT-homozygotes (p < 0.01, t-test). The difference in lymphocyte radiosensitivity between these 4 groups was also detected after in-vitro irradiation with 2 Gy (0.484 +/- 0.132 vs. 0.535 +/- 0.228 vs. 0.926 +/- 0.349 vs. 1.423 +/- 0.072). Conclusions: We found a significantly higher number of chromosomal breaks in lymphocytes of patients with severe or extreme radiation reaction of normal tissues as compared to patients with no or only mild to moderate radiation reactions. The radiosensitivity of lymphocytes in these radiosensitive patients was inthe range between normal radiosensitivity and the radiosensitivity ofAT-homozygotes. Detection of patients with severely enhanced intrinsic radiosensitivity might be possible with this method.

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Documento generato il 13/07/20 alle ore 06:09:23