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Titolo:
TUMOR-NECROSIS-FACTOR-ALPHA STIMULATES HUMAN CLARA CELL SECRETORY PROTEIN-PRODUCTION BY HUMAN AIRWAY EPITHELIAL-CELLS
Autore:
YAO XL; LEVINE SJ; COWAN MJ; LOGUN C; SHELHAMER JH;
Indirizzi:
NIH,DEPT CRIT CARE MED,CTR CLIN,BLDG 10,RM 7-F-43 BETHESDA MD 20892 NIH,DEPT CRIT CARE MED,CTR CLIN BETHESDA MD 20892
Titolo Testata:
American journal of respiratory cell and molecular biology
fascicolo: 4, volume: 19, anno: 1998,
pagine: 629 - 635
SICI:
1044-1549(1998)19:4<629:TSHCCS>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE-SPECIFIC EXPRESSION; GENE-EXPRESSION; PHOSPHOLIPASE A(2); RABBIT UTEROGLOBIN; BINDING-PROTEIN; POLYCHLORINATED-BIPHENYLS; PROGESTERONE-BINDING; NUCLEOTIDE-SEQUENCE; HORMONAL-REGULATION; ENDOTHELIAL-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
X.L. Yao et al., "TUMOR-NECROSIS-FACTOR-ALPHA STIMULATES HUMAN CLARA CELL SECRETORY PROTEIN-PRODUCTION BY HUMAN AIRWAY EPITHELIAL-CELLS", American journal of respiratory cell and molecular biology, 19(4), 1998, pp. 629-635

Abstract

Clara cell secretory protein (CCSP), or CC10, is an inhibitor of secretory phospholipase A(2) which may be produced by phagocytic cells andby a variety of other cells in the airway. Tumor necrosis factor-alpha (TNF-alpha) is capable of activating phospholipases and inducing theexpression of a variety of genes in the airway epithelium which may modulate the airway inflammatory response. Therefore, it was of interest to determine whether this proinflammatory cytokine could induce the production of a counterregulatory protein such as CCSP which might modulate the production of eicosanoid mediators in the airway. Using a human bronchial epithelial cell line (BEAS-2B), CCSP messenger RNA (mRNA) levels were detected by ribonuclease protection assay. TNF treatmentof these cells increased CCSP mRNA levels in a time- and dose-dependent manner. The CCSP mRNA level increased in response to TNF-alpha (20 ng/ml) stimulation after 8 to 36 h with the peak increase at 18 h. Immunoblotting of CCSP protein released into the culture media demonstrated that TNF-alpha induced the synthesis and secretion of CCSP protein in a time-dependent manner over 8 to 18 h. The results of a CCSP reporter gene activity assay, nuclear run-on assay, and CCSP mRNA half-lifeassay indicated that the TNF-alpha-induced increases in CCSP gene expression are regulated at the post-transcriptional level. We conclude that TNF-alpha induces airway epithelial cell expression of human CCSP protein and may modulate airway inflammatory responses in this manner.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:56:59