Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PBLA8, FROM BREVIBACTERIUM LINENS, BELONGS TO A GRAM-POSITIVE SUBFAMILY OF COLE2-RELATED PLASMIDS
Autore:
LERET V; TRAUTWETTER A; RINCE A; BLANCO C;
Indirizzi:
UNIV RENNES 1,UPRESA 6026,AVE GEN LECLERC F-35042 RENNES FRANCE UNIV RENNES 1,UPRESA 6026 F-35042 RENNES FRANCE
Titolo Testata:
Microbiology
, volume: 144, anno: 1998,
parte:, 10
pagine: 2827 - 2836
SICI:
1350-0872(1998)144:<2827:PFBLBT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOST-RANGE PLASMID; ACID-PRODUCING CORYNEBACTERIA; COMPLETE NUCLEOTIDE-SEQUENCE; ESCHERICHIA-COLI; ARTHROBACTER-AUREUS; CORYNEFORM BACTERIA; CLONING VECTORS; CRYPTIC PLASMID; LACTOFERMENTUM; REPLICATION;
Keywords:
BREVIBACTERIUM LINENS; PLASMID REPLICATION; COLE2 RELATED PLASMIDS; SHUTTLE VECTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
V. Leret et al., "PBLA8, FROM BREVIBACTERIUM LINENS, BELONGS TO A GRAM-POSITIVE SUBFAMILY OF COLE2-RELATED PLASMIDS", Microbiology, 144, 1998, pp. 2827-2836

Abstract

A 3.1 kb DNA fragment from pBLA8, a Brevibacterium linens cryptic plasmid, containing all the information required for autonomous replication was cloned and sequenced. Using deletion analysis, the fragment essential and sufficient for autonomous replication was delimited to 1.5 kb. This fragment is characterized by the presence of an ori site located upstream of an operon encoding two proteins, RepA and RepB, both essential for replication. Based on structural similarities and a strong conservation of ori, RepA and RepB, pBLA8 was assigned to a new subfamily of the ColE2 plasmid family. This subfamily is distinguished by the requirement for two Rep proteins and the location of an ori site upstream of the repAB operon. RepA is thought to encode primase activity, whereas RepB could be a DNA-binding protein. An Escherichia coli-B.linens shuttle vector, derived from pBLA8 was constructed. Its host spectrum was extended to Arthrobacter species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 19:04:32