Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHARACTERIZATION AND EXPRESSION OF A NEW CLASS OF ZINC-FINGER PROTEINTHAT BINDS TO SILENCER REGION OF ASCORBATE OXIDASE GENE
Autore:
KISU Y; ONO T; SHIMOFURUTANI N; SUZUKI M; ESAKA M;
Indirizzi:
HIROSHIMA UNIV,FAC APPL BIOL SCI,KAGAMIYAMA 1-4-4 HIGASHIHIROSHIMA 739 JAPAN HIROSHIMA UNIV,FAC APPL BIOL SCI HIGASHIHIROSHIMA 739 JAPAN AGCY IND SCI & TECHNOL,NIBHT STRUCT BIOL CTR TSUKUBA IBARAKI 305 JAPAN
Titolo Testata:
Plant and Cell Physiology
fascicolo: 10, volume: 39, anno: 1998,
pagine: 1054 - 1064
SICI:
0032-0781(1998)39:10<1054:CAEOAN>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTIONAL ACTIVATORS; MOLECULAR-CLONING; DNA; SEQUENCE; PROMOTER; MAIZE; MOTIF; HOMOLOGY; TOBACCO; PUMPKIN;
Keywords:
ASCORBATE OXIDASE (EC 1.10.3.3); DNA-BINDING PROTEIN; DOF/MOA DOMAIN; PUMPKIN (CUCURBITA SP.); ZINC FINGER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
Y. Kisu et al., "CHARACTERIZATION AND EXPRESSION OF A NEW CLASS OF ZINC-FINGER PROTEINTHAT BINDS TO SILENCER REGION OF ASCORBATE OXIDASE GENE", Plant and Cell Physiology, 39(10), 1998, pp. 1054-1064

Abstract

A unique A/T-rich sequence (5'-AAAAAGTAAAAAGTAAAAAAGTAAAAAG-3'), referred to as the AGTA repeat, is found in the silencer region of the pumpkin ascorbate oxidase gene. A cDNA for protein (AOBP) that binds to the AGTA repeat was isolated from pumpkin by the southwestern method. The AOBP protein has a new class of zinc/DNA-binding domain named Dof/MOA domain that is highly conserved in many plant proteins and is significantly related to those of steroid hormone receptors and GATA1. Gel retardation analysis indicated that AOBP bound to the AGTA repeat through the Dof/MOA domain. Metal chelators, I,10-phenanthroline and EDTA,specifically inhibited the DNA binding of AOBP, indicating that metalcoordination plays an important role in DNA binding of AOBP, Thus, the Dof/MOA domain acts as a zinc/DNA-binding domain in AOBP, Gel retardation analysis with mutated oligonucleotides suggested that the Dof/MOA domain recognized the AGTA core sequence. AOBP mRNA was expressed inmature tissues of pumpkin, but was expressed only in small amounts orwas not expressed in growing tissues. Furthermore, the expression wasauxin-independent. The expression pattern of AOBP and that of ascorbate oxidase did not show a positive correlation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:34:12