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Titolo:
2 TYPES OF BRAIN CHONDROITIN SULFATE PROTEOGLYCAN - THEIR DISTRIBUTION AND POSSIBLE FUNCTIONS IN THE RAT EMBRYO
Autore:
KATOHSEMBA R; MATSUDA M; WATANABE E; MAEDA N; OOHIRA A;
Indirizzi:
AICHI HUMAN SERV CTR,INST DEV RES,DEPT PERINATOL KASUGAI AICHI 4800392 JAPAN AICHI HUMAN SERV CTR,INST DEV RES,DEPT EMBRYOL KASUGAI AICHI 4800392 JAPAN NATL INST BASIC BIOL,DEPT MOL NEUROBIOL OKAZAKI AICHI 4448585 JAPAN
Titolo Testata:
Neuroscience research
fascicolo: 4, volume: 31, anno: 1998,
pagine: 273 - 282
SICI:
0168-0102(1998)31:4<273:2TOBCS>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; EXTRACELLULAR-MATRIX; NEURITE OUTGROWTH; CELL-ADHESION; AXON GROWTH; PC12D CELLS; NEUROCAN; PROTEINS; PHOSPHACAN; BARRIERS;
Keywords:
BRAIN; CHONDROITIN SULFATE; PC12 CELLS; PROTEOGLYCAN; RAT EMBRYO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
R. Katohsemba et al., "2 TYPES OF BRAIN CHONDROITIN SULFATE PROTEOGLYCAN - THEIR DISTRIBUTION AND POSSIBLE FUNCTIONS IN THE RAT EMBRYO", Neuroscience research, 31(4), 1998, pp. 273-282

Abstract

The distribution of neurocan-like and 6B4 proteoglycan-like immunoreactivities in the rat embryo was investigated from gestational days 10.5-15.5 with monoclonal antibody 1G2 or 6B4 that immunoreacted with neurocan and 6B4 proteoglycan, respectively. In the brain region, the leptomeningeal layer in the myelencephalon, metencephalon, diencephalon or telencephalon was first stained with monoclonal antibody 1G2 at embryonic day 12.5. In the spinal cord, monoclonal antibody 1G2 stained the regions corresponding to the boundary caps (designated the boundary caps) after embryonic day 11.5 and the roof plate after embryonic day 12.5. The intensity of staining in the boundary caps reached a maximumat embryonic day 13.5, at around the time when the axons from the dorsal root ganglia reach this region. However, the points of contact of the axons with the boundary caps were hardly stained. By contrast, theroof plate was most strongly and widely stained at embryonic day 14.5, at around the time when the axons enter the spinal cord. Western blotting of preparations from the spinal cord that included the boundary caps revealed the presence of neurocan in this region. Thus, it is likely that neurocan serves as a barrier molecule to regulate the direction of axonal growth from the dorsal root ganglia. By contrast, in addition to staining of the future brain and spinal cord, monoclonal antibody 6B4 stained the trigeminal and sympathetic ganglia in the rat embryo on and after embryonic day 12.5, as well as the vestibular, facial and dorsal root ganglia after embryonic day 12.5. In studies in tissueculture, monoclonal antibody 6B4 prevented the inhibitory effects of 6B4 proteoglycan on the proliferation of PC12D cells. No immunostaining with monoclonal antibody 6B4 was observed in cells that had incorporated bromodeoxyuridine in vivo. Possible functions of 6B4 proteoglycanin the rat embryo are discussed. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 18:09:59