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Titolo:
CELL-MEDIATED OXIDATION OF LDL - COMPARISON OF DIFFERENT CELL-TYPES OF THE ATHEROSCLEROTIC LESION
Autore:
MULLER K; CARPENTER KLH; MITCHINSON MJ;
Indirizzi:
UNIV CAMBRIDGE,DEPT PATHOL,DIV CELLULAR PATHOL,TENNIS COURT RD CAMBRIDGE CB2 1QP ENGLAND
Titolo Testata:
Free radical research (Print)
fascicolo: 3, volume: 29, anno: 1998,
pagine: 207 - 220
SICI:
1071-5762(1998)29:3<207:COOL-C>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HUMAN MONOCYTE-MACROPHAGES; SMOOTH-MUSCLE CELLS; LIPID-PEROXIDATION; ENDOTHELIAL-CELLS; VITAMIN-E; INVITRO; CULTURE; METABOLISM; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
K. Muller et al., "CELL-MEDIATED OXIDATION OF LDL - COMPARISON OF DIFFERENT CELL-TYPES OF THE ATHEROSCLEROTIC LESION", Free radical research (Print), 29(3), 1998, pp. 207-220

Abstract

The three major cell types of the human atherosclerotic lesion - macrophages (Mo), smooth muscle cells (SMC) and endothelial cells (EC) - were compared for their ability to oxidise low density lipoprotein (LDL) in vitro under identical conditions. Near-confluent cultures were incubated for up to 48 h with 50 mu g protein/ml LDL in Ham's F10 mediumsupplemented with 7 mu M Fe2+. All three cell types oxidised LDL readily using our culture conditions. After 24 and 48 h, the degree of LDLoxidation was in the order: Mo > SMC > EC when based on cell growth area and EC > SMC > Ms when based on cellular DNA content. However, LDLoxidation in vitro progressed more slowly between 24 and 48 h, probably due to increasing toxicity to the cells and/or depletion of polyunsaturated fatty acids. We therefore compared the time of onset of LDL oxidation. The earliest increase in LDL oxidation was always apparent with SMC. Gas chromatography revealed that LDL oxidation by all three cell types followed a similar pattern. The polyunsaturated fatty acids linoleic acid (18:2) and arachidonic acid (20:4) were depleted (to 10.3-18.1% and 4.5-24.7% respectively, compared to native LDL), whereas the content of stearic acid (18:0) and oleic acid (18:1) remained unchanged. Cholesterol was depleted (to 54.1-75.6% of native LDL) with a concomitant rise in 7 beta-hydroxycholesterol (to 60.6-128.1 mu g/mg LDL). This corresponds to a conversion of 4.9, 9.5 and 10.4% of LDL cholesterol in EC-, SMC- and Mo-modified LDL respectively. All three cell types showed significant toxicity in the oxidising culture after 24 h. The possible relevance to LDL oxidation in atherosclerosis is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/02/20 alle ore 12:35:09