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Titolo:
CHARACTERIZATION OF LA-CROSSE VIRUS-RNA IN AUTOPSIED CENTRAL-NERVOUS-SYSTEM TISSUES
Autore:
CHANDLER LJ; BORUCKI MK; DOBIE DK; WASIELOSKI LP; THOMPSON WH; GUNDERSEN CB; CASE K; BEATY BJ;
Indirizzi:
COLORADO STATE UNIV,COLL VET MED & BIOMED SCI,DEPT MICROBIOL,ARTHROPOD & INFECT DIS LAB FT COLLINS CO 80523 COLORADO STATE UNIV,COLL VET MED & BIOMED SCI,DEPT MICROBIOL,ARTHROPOD & INFECT DIS LAB FT COLLINS CO 80523 GUNDERSEN LUTHERAN MED CTR LA CROSSE WI 54601 UNIV WISCONSIN,DEPT PREVENT MED MADISON WI 53706
Titolo Testata:
Journal of clinical microbiology (Print)
fascicolo: 11, volume: 36, anno: 1998,
pagine: 3332 - 3336
SICI:
0095-1137(1998)36:11<3332:COLVIA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MEASLES-VIRUS; CEREBROSPINAL-FLUID; LACROSSE VIRUS; UNITED-STATES; PCR; ENCEPHALITIS; INFECTIONS; DIAGNOSIS; BUNYAVIRUSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
L.J. Chandler et al., "CHARACTERIZATION OF LA-CROSSE VIRUS-RNA IN AUTOPSIED CENTRAL-NERVOUS-SYSTEM TISSUES", Journal of clinical microbiology (Print), 36(11), 1998, pp. 3332-3336

Abstract

A reverse transcription-PCR (RT-PCR) technique was used to detect La Crosse (LAC) virus RNA in the central nervous system (CNS) tissues of two patients who died of LAC encephalitis in 1960 and 1978, Viral RNA was readily detected by RT-PCR although the tissues had been stored frozen for up to 37 years. LAC virus was detected in the cerebral cortexbut not in other CNS tissues. RT-PCR allowed detection of replicativeforms of the virus, indicating that the virus was actively replicating in the specific CNS tissues. The small (S) RNA segments of the viruses from the CNS samples were demonstrated to be genetically similar bysingle-strand conformation polymorphism analyses. These S RNA segments were then sequenced; only two base changes were demonstrated betweenthe 1960 and the 1978 samples, suggesting that LAC virus is genetically stable in areas of endemicity, The RT-PCR analyses of analyte directly from CNS tissues allows study of the virus without passage in cellculture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:15:10