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Titolo:
DIAGNOSTIC POTENTIAL OF TOSCANA VIRUS N-PROTEIN EXPRESSED IN ESCHERICHIA-COLI
Autore:
VALASSINA M; SOLDATESCHI D; DALMASO GM; SANTINI L; BIANCHI S; VALENSIN PE; CUSI MG;
Indirizzi:
UNIV SIENA,DEPT MOL BIOL,MICROBIOL SECT I-53100 SIENA ITALY UNIV SIENA,DEPT MOL BIOL,MICROBIOL SECT I-53100 SIENA ITALY DIESSE SRL I-53035 MONTERIGGIONI SI ITALY
Titolo Testata:
Journal of clinical microbiology (Print)
fascicolo: 11, volume: 36, anno: 1998,
pagine: 3170 - 3172
SICI:
0095-1137(1998)36:11<3170:DPOTVN>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVOUS-SYSTEM INVOLVEMENT; SANDFLY FEVER VIRUS; NUCLEOCAPSID PROTEINS; SEROTYPE TOSCANA; PHLEBOVIRUS; INFECTION; SEQUENCE; SEGMENT; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
M. Valassina et al., "DIAGNOSTIC POTENTIAL OF TOSCANA VIRUS N-PROTEIN EXPRESSED IN ESCHERICHIA-COLI", Journal of clinical microbiology (Print), 36(11), 1998, pp. 3170-3172

Abstract

The nucleocapsid (N) protein of the Toscana (TOS) virus was expressedin Escherichia coli by using a pET15b vector. The recombinant proteinwas purified by affinity chromatography and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting,and enzyme immunoassay (EIA), The recombinant antigen was reactive with positive human sera, and the reactivity correlated very well (r = 0.9) with that of a whole-virus antigen when tested by EIA with 30 TOS virus-positive and 30 TOS virus-negative serum samples. The results demonstrate that the recombinant N protein can be easily produced in a procaryotic system and used for diagnostic assays for TOS virus immunity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:04:44