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Titolo:
HUMAN VASCULAR ENDOTHELIAL-CELLS EXPRESS FUNCTIONAL NICOTINIC ACETYLCHOLINE-RECEPTORS
Autore:
MACKLIN KD; MAUS ADJ; PEREIRA EFR; ALBUQUERQUE EX; CONTIFINE BM;
Indirizzi:
UNIV MINNESOTA,DEPT BIOCHEM,1479 GORTNER AVE ST PAUL MN 55108 UNIV MINNESOTA,DEPT BIOCHEM ST PAUL MN 55108 UNIV MINNESOTA,DEPT PHARMACOL MINNEAPOLIS MN 55455 UNIV MARYLAND,SCH MED,DEPT PHARMACOL & EXPT THERAPEUT BALTIMORE MD 21201
Titolo Testata:
The Journal of pharmacology and experimental therapeutics
fascicolo: 1, volume: 287, anno: 1998,
pagine: 435 - 439
SICI:
0022-3565(1998)287:1<435:HVEEFN>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN KERATINOCYTES; SMOOTH-MUSCLE; SUBUNITS; RAT; SUBTYPES; RELEASE; MICROVESSELS; LOCALIZATION; NEURONS; ALPHA-5;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
K.D. Macklin et al., "HUMAN VASCULAR ENDOTHELIAL-CELLS EXPRESS FUNCTIONAL NICOTINIC ACETYLCHOLINE-RECEPTORS", The Journal of pharmacology and experimental therapeutics, 287(1), 1998, pp. 435-439

Abstract

ACh receptors sensitive to nicotine (nAChR) are present in human skinkeratinocytes and in bronchial epithelial cells. They are stimulated by ACh secreted by the same cells that express them, and they modulatecell motility and shape. A variety of non-neuronal tissues, includingendothelial cells, synthesize ACh, which raises the possibility that they are sensitive to nicotine. We demonstrate here that endothelial cells that line blood vessels express functional nAChRs. Their structure and ion-gating properties are similar to those of the nAChRs expressed by ganglionic neurons and by skin keratinocytes and bronchial epithelial cells. In situ hybridization experiments using primary cultures of endothelial cells from human aorta demonstrated the presence in these cells of the subunits believed to contribute to ganglionic ACh receptors (AChRs) of the alpha 3 subtype: alpha 3, alpha 5, beta 2 and beta 4. Binding of radiolabeled epibatidine-a high-affinity specific ligand of certain neuronal AChRs, including the alpha 3 subtypes-revealed the presence of approximately 900 specific binding sites per cell. We assessed the presence of functional AChRs by patch-clamp experiments. Cultured human endothelial cells express ion channels that are opened by (+)-anatoxin-a and are blocked by dihydro-beta-erythroidine. These are specific agonist and antagonist, respectively, of neuronal AChRs of the alpha 3 subtype. The ion-gating properties of the endothelial AChRs were similar to those of neuronal ganglionic AChRs. The presence of AChRs sensitive to nicotine in endothelial cells may be related to the toxic effects of nicotine on the vascular system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:09:41