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Titolo:
CRYOPRESERVATION OF TRANSFORMED PAPAVER-SOMNIFERUM CELLS
Autore:
GAZEAU C; ELLEUCH H; DAVID A; MORISSET C;
Indirizzi:
UNIV PARIS 06,LAB CRYOBIOL VEGETALE,12 RUE CUVIER F-75252 PARIS 05 FRANCE UNIV PICARDIE,FAC SCI,LAB BIOTECHNOL & PHYSIOL VEGETALES F-80039 AMIENS FRANCE
Titolo Testata:
Cryo-letters
fascicolo: 3, volume: 19, anno: 1998,
pagine: 147 - 158
SICI:
0143-2044(1998)19:3<147:COTPC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
AXILLARY SHOOT TIPS; ENCAPSULATION-DEHYDRATION; SUSPENSION CULTURES; CATHARANTHUS-ROSEUS; INVITRO PLANTLETS; LIQUID-NITROGEN; VITRIFICATION; POLYAMINES; RESISTANCE;
Keywords:
CRYOPRESERVATION; PAPAVER SOMNIFERUM; ENCAPSULATION; CELL SUSPENSION; TRANSGENIC CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
C. Gazeau et al., "CRYOPRESERVATION OF TRANSFORMED PAPAVER-SOMNIFERUM CELLS", Cryo-letters, 19(3), 1998, pp. 147-158

Abstract

Different procedures were tested in an attempt to cryopreserve transformed cells of Papaver somniferum in liquid nitrogen. The encapsulation-dehydration process yielded negative results. Cells were encapsulated in alginate beads and precultured for 5 days in 0.75M maltose or sucrose-enriched medium. They survived a 3-h dehydration, but not freezing to -196 degrees C. The vitrification method, which needed highly concentrated solutions, also proved ineffective for embedded and non-embedded cells. In contrast, using the conventional procedure, suspension cultures resisted to -196 degrees C, when treated with 0.75M sorbitol or maltose and 5% DMSO, and two-step frozen. Better results after freezing were achieved using an original method including encapsulation ofcells and conventional cryopreservation. This procedure based on trapping of cells in calcium-alginate allowed easier handling faster regrowth than for non-encapsulated cells, and successful cryopreservation at -196 degrees C of Papaver suspensions after a cryoprotective treatment combined with progressive freezing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:00:00