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Titolo:
STAT3 ACTIVATION ACCOMPANIES KERATINOCYTE DIFFERENTIATION
Autore:
HAUSER PJ; AGRAWAL D; HACKNEY J; PLEDGER WJ;
Indirizzi:
UNIV S FLORIDA,COLL MED,H LEE MOFFITT CANC CTR & RES INST,12902 MAGNOLIA DR TAMPA FL 33612 UNIV S FLORIDA,COLL MED,H LEE MOFFITT CANC CTR & RES INST TAMPA FL 33612 UNIV S FLORIDA,COLL MED,DEPT MICROBIOL TAMPA FL 33612 UNIV S FLORIDA,COLL MED,DEPT BIOCHEM & MOL BIOL TAMPA FL 33612 VANDERBILT UNIV,DEPT CELL BIOL NASHVILLE TN 37240
Titolo Testata:
Cell growth & differentiation
fascicolo: 10, volume: 9, anno: 1998,
pagine: 847 - 855
SICI:
1044-9523(1998)9:10<847:SAAKD>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR CALCIUM CONCENTRATIONS; STRATIFIED SQUAMOUS EPITHELIA; DEPENDENT KINASE-ACTIVITY; HUMAN EPIDERMAL-CELLS; TERMINAL DIFFERENTIATION; GROWTH-FACTOR; IN-VITRO; MURINE KERATINOCYTES; RECEPTOR KINASE; GENE-REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
P.J. Hauser et al., "STAT3 ACTIVATION ACCOMPANIES KERATINOCYTE DIFFERENTIATION", Cell growth & differentiation, 9(10), 1998, pp. 847-855

Abstract

The signal transducers and activators of transcription (STAT) family of transcription factors has been demonstrated to play key roles in a variety of cell types under conditions that promote differentiation orcell cycle exit. We report our studies of primary murine keratinocytes in which we demonstrate activation of STAT3 during growth arrest anddifferentiation. In adherent cells, STAT3-specific DNA binding activity was detected in quiescent cultures, down-regulated upon mitogenic stimulation, and found to reaccumulate as cells reentered quiescence. Suspension culturing of proliferating keratinocytes, which induces differentiation, also resulted in induction of STAT3 activity. Furthermore, induction of STAT3 after suspension culturing did not occur in MK cells, an immortalized murine keratinocyte cell line that does not undergo differentiation. Because STAT3 activation in these cells corresponded tightly with the growth status, we examined whether there was a relationship between the cell cycle machinery and STAT3 activation by inhibiting p27(kip1) accumulation, which is observed during growth arrest, with antisense oligonucleotides and by using keratinocytes lacking functional p27(kip1). In both cases, there was a loss of STAT3 activation and a concomitant delay in terminal cell cycle withdrawal and in the expression of the differentiation specific marker, keratin 1. Thus, in addition to controlling transcription mediated through E2F, our data demonstrate that alterations in the cell cycle machinery are required for appropriate up-regulation of STAT3 activity that occurs during keratinocyte differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 08:10:48