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Titolo:
CUT11(- A GENE REQUIRED FOR CELL CYCLE-DEPENDENT SPINDLE POLE BODY ANCHORING IN THE NUCLEAR-ENVELOPE AND BIPOLAR SPINDLE FORMATION IN SCHIZOSACCHAROMYCES-POMBE() )
Autore:
WEST RR; VAISBERG EV; DING R; NURSE P; MCINTOSH JR;
Indirizzi:
UNIV COLORADO,DEPT MOL CELLULAR & DEV BIOL BOULDER CO 80309
Titolo Testata:
Molecular biology of the cell
fascicolo: 10, volume: 9, anno: 1998,
pagine: 2839 - 2855
SICI:
1059-1524(1998)9:10<2839:CAGRFC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
FISSION YEAST; SACCHAROMYCES-CEREVISIAE; CHROMOSOME MOVEMENT; ELECTRON-MICROSCOPY; SEPTUM FORMATION; PROTEIN-KINASE; PORE COMPLEX; MITOSIS; DIVISION; MUTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
R.R. West et al., "CUT11(- A GENE REQUIRED FOR CELL CYCLE-DEPENDENT SPINDLE POLE BODY ANCHORING IN THE NUCLEAR-ENVELOPE AND BIPOLAR SPINDLE FORMATION IN SCHIZOSACCHAROMYCES-POMBE() )", Molecular biology of the cell, 9(10), 1998, pp. 2839-2855

Abstract

The ''cut'' mutants of Schizosaccharomyces pombe are defective in spindle formation and/or chromosome segregation, but they proceed throughthe cell cycle, resulting in lethality. Analysis of temperature-sensitive alleles of cut11(+) suggests that this gene is required for the formation of a functional bipolar spindle. Defective spindle structure was revealed with fluorescent probes for tubulin and DNA. Three-dimensional reconstruction of mutant spindles by serial sectioning and electron microscopy showed that the spindle pole bodies (SPBs) either failed to complete normal duplication or were free floating in the nucleoplasm. Localization of Cut11p tagged with the green fluorescent protein showed punctate nuclear envelope staining throughout the cell cycle and SPBs staining from early prophase to mid anaphase. This SPB localization correlates with the time in the cell cycle when SPBs are insertedinto the nuclear envelope. Immunoelectron microscopy confirmed the localization of Cut11p to mitotic SPBs and nuclear pore complexes. Cloning and sequencing showed that cut11(+) encodes a novel protein with seven putative membrane-spanning domains and homology to the Saccharomyces cerevisiae gene NDC1. These data suggest that Cut11p associates with nuclear pore complexes and mitotic SPBs as an anchor in the nuclear envelope; this role is essential for mitosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 10:45:45