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Titolo:
ISOLATION AND CHARACTERIZATION OF THE FEMALE-SPECIFIC PROTEIN (VITELLOGENIN) IN MATURE FEMALE HEMOLYMPH OF THE PRAWN PENAEUS-CHINENSIS
Autore:
CHANG CF; JENG SR;
Indirizzi:
NATL TAIWAN OCEAN UNIV,DEPT AQUACULTURE CHILUNG 20224 TAIWAN NATL KAOSHIUNG JR COLL MARINE TECHNOL,DEPT AQUACULTURE KAOHSIUNG 81105 TAIWAN
Titolo Testata:
Comparative biochemistry and physiology. B. Comparative biochemistry
fascicolo: 2, volume: 112, anno: 1995,
pagine: 257 - 263
SICI:
0305-0491(1995)112:2<257:IACOTF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACROBRACHIUM-ROSENBERGII; MALACOSTRACAN CRUSTACEA; ARMADILLIDIUM-VULGARE; TERRESTRIAL ISOPOD; CARCINUS-MAENAS; VITELLIN; OVARY; PURIFICATION; LIPOVITELLIN; VANNAMEI;
Keywords:
FEMALE-SPECIFIC PROTEIN; HEMOLYMPH; ISOLATION; PENNEUS CHINENSIS; PRAWN; VITELLOGENIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
C.F. Chang e S.R. Jeng, "ISOLATION AND CHARACTERIZATION OF THE FEMALE-SPECIFIC PROTEIN (VITELLOGENIN) IN MATURE FEMALE HEMOLYMPH OF THE PRAWN PENAEUS-CHINENSIS", Comparative biochemistry and physiology. B. Comparative biochemistry, 112(2), 1995, pp. 257-263

Abstract

Purifying and characterizing the female-specific protein (vitellogenin) from the hemolymph of mature female prawns, Penaeus chinensis, werethe objectives of this study, Hemolymph was stained with Sudan Black B and fractionated by an ultracentrifuge. A specific protein (vitellogenin) appeared in the third fraction of the female hemolymph, based onpolyacrylamide gel electrophoresis (PAGE), Western blotting and immunoprecipitation. A purified vitellogenin was obtained from PAGE and electroelution. Two polypeptide subunits (191 and 85 kDa) were detected in SDS-PAGE, The purified vitellogenin was considered as a lipoglycophosphoprotein on the basis of staining data. Amino acid composition was also determined in the purified vitellogenin after acid hydrolysis. A very low level of cysteine was detected, Non-disulfide bonds were found in the binding of polypeptide subunits.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 02:39:57