Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHARACTERIZATION OF YOLK PROTEINS DURING OOCYTE DEVELOPMENT OF TILAPIA, OREOCHROMIS-MOSSAMBICUS
Autore:
JOHANNING KM; SPECKER JL;
Indirizzi:
UNIV RHODE ISL,DEPT ZOOL KINGSTON RI 02881 UNIV RHODE ISL,DEPT ZOOL KINGSTON RI 02881
Titolo Testata:
Comparative biochemistry and physiology. B. Comparative biochemistry
fascicolo: 2, volume: 112, anno: 1995,
pagine: 177 - 189
SICI:
0305-0491(1995)112:2<177:COYPDO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TROUT SALMO-GAIRDNERI; VITELLINE ENVELOPE PROTEINS; SODIUM DODECYL-SULFATE; EGG-YOLK; RAINBOW-TROUT; VITELLOGENIN RECEPTOR; GEL-ELECTROPHORESIS; STRUCTURAL PROTEINS; FUNDULUS OOCYTES; SERUM-PROTEIN;
Keywords:
TILAPIA (OREOCHROMIS MOSSAMBICUS); YOLK PROTEINS; VITELLOGENIN; OOCYTE; LIPOVITELLINS; PHOSVITINS; PROTEOLYTIC CLEAVAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
K.M. Johanning e J.L. Specker, "CHARACTERIZATION OF YOLK PROTEINS DURING OOCYTE DEVELOPMENT OF TILAPIA, OREOCHROMIS-MOSSAMBICUS", Comparative biochemistry and physiology. B. Comparative biochemistry, 112(2), 1995, pp. 177-189

Abstract

Comparisons of yolk proteins in tilapia, Oreochromis mossambicus, during oocyte development indicate differences between early and late vitellogenic stages. Changes during oocyte development were observed in the abundance of yolk proteins and in the immunoreactivity to VTG-200 and VTG-130 antibodies. Some of these yolk proteins were observed at early vitellogenic stages, 145, 48, 44, 42, 32, 32, 30, 11.7, and 11.5 kDa; others were observed at late vitellogenic stages, 87 and 64 kDa; whereas some were observed at both vitellogenic stages, 116, 105, 78, 22, 36, 25.5, 18, 16.5, 14, 13.8, and 12 kDa. Immunological analysis and staining with Coomassie Brilliant Blue indicate that VTG-130 appearsintact in the oocytes only at early stages. Intact VTG-200 is presentin both early and late oocyte developmental stages. Crossreaction of the two different antibodies with smaller molecular mass proteins fromthe oocytes suggests that they are proteolytic cleavage products of the vitellogenins. Phosvitins such as 21, 17, 13.5, and 8 kDa and lipovitellins measuring 116, 105, 18, 16.5, and 14 kDa were observed throughout oocyte development. Several yolk proteins, including 105, 87, 78,14, and 13.8 kDa, crossreacted with wheat germ agglutinin and concanavalin A lectins, suggesting that these contain N-acetylglucosamine andglucose and/or mannose.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:50:53