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Titolo:
NITROGEN AND OXYGEN REGULATION OF BACILLUS-SUBTILIS NASDEF ENCODING NADH-DEPENDENT NITRITE REDUCTASE BY TNRA AND RESDE
Autore:
NAKANO MM; HOFFMANN T; ZHU Y; JAHN D;
Indirizzi:
OREGON GRAD INST SCI & TECHNOL,DEPT BIOCHEM & MOL BIOL,POB 91000 PORTLAND OR 97291 LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOL BIOL SHREVEPORT LA 71130 UNIV FREIBURG,INST ORGAN CHEM & BIOCHEM D-79104 FREIBURG GERMANY MAX PLANCK INST TERR MIKROBIOL D-35043 MARBURG GERMANY
Titolo Testata:
Journal of bacteriology (Print)
fascicolo: 20, volume: 180, anno: 1998,
pagine: 5344 - 5350
SICI:
0021-9193(1998)180:20<5344:NAOROB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRATE ASSIMILATION; NASB OPERON; GENE; IDENTIFICATION; ACTIVATION; GROWTH; SYSTEM; FNR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
M.M. Nakano et al., "NITROGEN AND OXYGEN REGULATION OF BACILLUS-SUBTILIS NASDEF ENCODING NADH-DEPENDENT NITRITE REDUCTASE BY TNRA AND RESDE", Journal of bacteriology (Print), 180(20), 1998, pp. 5344-5350

Abstract

The nitrate and nitrite reductases of Bacillus subtilis have two different physiological functions. Under conditions of nitrogen limitation, these enzymes catalyze the reduction of nitrate via nitrite to ammonia for the anabolic incorporation of nitrogen into biomolecules. They also function catabolically in anaerobic respiration, which involves the use of nitrate and nitrite as terminal electron accepters. Two distinct nitrate reductases, encoded by narGHI and nasBC, function in anabolic and catabolic nitrogen metabolism, respectively. However, as reported herein, a single NADH-dependent, soluble nitrite reductase encoded by the nasDE genes is required for both catabolic and anabolic processes. The nasDE genes, together with nasBC (encoding assimilatory nitrate reductase) and nasF (required for nitrite reductase siroheme cofactor formation), constitute the nas operon, Data presented show that transcription of nasDEF is driven not only by the previously characterized nas operon promoter but also hom an internal promoter residing between the nasC and nasD genes. Transcription from both promoters is activated by nitrogen limitation during aerobic growth by the nitrogen regulator, TnrA However, under conditions of oxygen limitation, nasDEF expression and nitrite reductase activity were significantly induced. Anaerobic induction of nasDEF required the ResDE two-component regulatory system and the presence of nitrite, indicating partial coregulation of NasDEF with the respiratory nitrate reductase NarGHI during nitraterespiration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:37:38