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Titolo:
REGULATION OF NUCLEOSIDE TRANSPORT BY LIPOPOLYSACCHARIDE, PHORBOL ESTERS, AND TUMOR-NECROSIS-FACTOR-ALPHA IN HUMAN B-LYMPHOCYTES
Autore:
SOLER C; FELIPE A; MATA JF; CASADO FJ; CELADA A; PASTORANGLADA M;
Indirizzi:
UNIV BARCELONA,DEPT BIOQUIM & BIOL MOL,INST INVEST BIOMED AUGUST PI &SUNYER,AVDA DIAGONAL 645 BARCELONA 08071 SPAIN UNIV BARCELONA,DEPT BIOQUIM & BIOL MOL,INST INVEST BIOMED AUGUST PI &SUNYER BARCELONA 08071 SPAIN UNIV BARCELONA,DEPT FISIOL IMMUNOL E-08007 BARCELONA SPAIN UNIV BARCELONA,FDN PI & SUNYER E-08007 BARCELONA SPAIN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 41, volume: 273, anno: 1998,
pagine: 26939 - 26945
SICI:
0021-9258(1998)273:41<26939:RONTBL>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; GUANINE RIBONUCLEOSIDES; FUNCTIONAL EXPRESSION; URIDINE-TRANSPORT; LEUKEMIA-CELLS; HL-60 CELLS; RAT-LIVER; ACTIVATION; MOUSE; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
C. Soler et al., "REGULATION OF NUCLEOSIDE TRANSPORT BY LIPOPOLYSACCHARIDE, PHORBOL ESTERS, AND TUMOR-NECROSIS-FACTOR-ALPHA IN HUMAN B-LYMPHOCYTES", The Journal of biological chemistry, 273(41), 1998, pp. 26939-26945

Abstract

Nucleoside transport systems and their regulation in human B-lymphocytes have been characterized using the cell lines Raji and Bare lymphoma syndrome-1 (BLS-1) as experimental models. These cells express at least three different nucleoside transport systems as follows: a nitrobenzylthioinosine-sensitive equilibrative transport system of the es-type, which appears to be associated with hENT1 expression, and two Na+-dependent transport systems that may correspond to N1 and to the recently characterized NS-type, which is nitrobenzylthioinosine-sensitive and guanosine-preferring. B cell activators such as phorbol 12-myristate13-acetate and lipopolysaccharide (LPS) up-regulate both concentrative transport systems but down-regulate the equilibrative es-type transporter, which correlates with lower hENT1 mRNA levels. These effects are dependent on protein kinase C activity. Phorbol 12-myristate 13-acetate and LPS also induce an increase in tumor necrosis factor-alpha (TNF-alpha) mRNA levels, which suggest that this cytokine may mediate some of the effects triggered by these agents, since addition of TNF-alpha alone can increase N1 and N5 transport activities by a mechanism that also depends on protein kinase C activation. Interestingly, TNF-alpha down-regulates es activity, but this effect cannot be abolished by inhibiting protein kinase C, This study reveals differential regulationof nucleoside transport systems following activation of human B-lymphocyte cell lines by agents of physiological relevance such as TNF-alpha and LPS, Moreover, it indicates that the recently characterized N5 transport system can also be regulated following B cell activation, which may be relevant to lymphocyte physiology and to the treatment of lymphocyte malignancies.

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Documento generato il 07/07/20 alle ore 05:07:03