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Titolo:
EXPRESSION AND TRANSSYNAPTIC REGULATION OF P-2X4 AND P-2Z RECEPTORS FOR EXTRACELLULAR ATP IN PAROTID ACINAR-CELLS - EFFECTS OF PARASYMPATHETIC DENERVATION
Autore:
TENNETI L; GIBBONS SJ; TALAMO BR;
Indirizzi:
TUFTS UNIV,SCH MED,DEPT NEUROSCI,136 HARRISON AVE BOSTON MA 02111 TUFTS UNIV,SCH MED,DEPT NEUROSCI BOSTON MA 02111
Titolo Testata:
The Journal of biological chemistry
fascicolo: 41, volume: 273, anno: 1998,
pagine: 26799 - 26808
SICI:
0021-9258(1998)273:41<26799:EATROP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT SALIVARY-GLANDS; GATED ION CHANNELS; SUBSTANCE-P; MAST-CELLS; AMYLASE SECRETION; SUPER-SENSITIVITY; CYTOSOLIC CALCIUM; SENSORY NEURONS; P2X(7) RECEPTOR; PLASMA-MEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
L. Tenneti et al., "EXPRESSION AND TRANSSYNAPTIC REGULATION OF P-2X4 AND P-2Z RECEPTORS FOR EXTRACELLULAR ATP IN PAROTID ACINAR-CELLS - EFFECTS OF PARASYMPATHETIC DENERVATION", The Journal of biological chemistry, 273(41), 1998, pp. 26799-26808

Abstract

Trans-synaptic regulation of muscarinic, peptidergic, and purinergic responses after denervation has been reported previously in rat parotid acinar cells (McMillian, M. K., Soltoff, S. P., Cantley, L. C., Rudel, R., and Talamo, B. R. (1993) Br. J. Pharmacol. 108, 453-461), Characteristics of the ATP-mediated responses and the effects of parasympathetic denervation were further analyzed through assay of Ca2+ influx, using fluorescence ratio imaging methods, and by analysis of P-2x receptor expression. ATP activates both a high affinity and a low affinityresponse with properties corresponding to the recently described P-2x4 and the P-2z (P-2x7)-type purinoceptors, respectively. Reverse transcription-polymerase chain reaction analysis reveals mRNA for P-2x4 as well as P-2x7 subtypes but not P-2x1, P-2x2, P-2x3, P-2x5, or P-2x6. P-2x4 protein also is detected by Western blotting. Distribution of thetwo types of ATP receptor responses on individual cells was stochastic, with both high and low affinity responses on some cells, and only asingle type of response on others. Sensitivity to P-2x4-type activation also varied even among cells responsive to low concentrations of ATP. Parasympathetic denervation greatly enhanced responses, tripling the proportion of acinar cells with a P-2x4-type response and increasingthe fraction of highly sensitive cells by 7-fold, Moreover, P-2x4 mRNA is significantly increased following parasympathetic denervation. These data indicate that sensitivity to ATP is modulated by neurotransmission at parasympathetic synapses, at least in part through increased expression of P-2x4 mRNA, and suggest that similar regulation may occur at other sites in the nervous system where P-2x4 receptors are widely expressed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:41:51