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Titolo:
The chemokine monocyte chemoattractant protein-1 induces functional responses through dimerization of its receptor CCR2
Autore:
Rodriguez-Frade, JM; Vila-Coro, AJ; de Ana, AM; Albar, JP; Martinez-A, C; Mellado, M;
Indirizzi:
Univmmunoloma Madrid, Consejo Super Inves Cient, Ctr Nacl Biotecnol, Dept I Univ Autonoma Madrid Madrid Spain E-28049 nt, Ctr Nacl Biotecnol, Dept I
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 7, volume: 96, anno: 1999,
pagine: 3628 - 3633
SICI:
0027-8424(19990330)96:7<3628:TCMCPI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-TERMINAL DOMAIN; SIGNAL-TRANSDUCTION; MEDIATED ACTIVATION; HIV-1 INFECTION; IMMUNE-RESPONSE; MAP-KINASE; T-CELLS; MECHANISM; RANTES; NEUTROPHILS;
Keywords:
chemotaxis; inflammation; HIV type 1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Martinez-A, C Univmmunoloma Madrid, Consejo Super Inves Cient, Ctr Nacl Biotecnol, Dept I Univ Autonoma Madrid Campus Cantoblanco Madrid Spain E-28049
Citazione:
J.M. Rodriguez-Frade et al., "The chemokine monocyte chemoattractant protein-1 induces functional responses through dimerization of its receptor CCR2", P NAS US, 96(7), 1999, pp. 3628-3633

Abstract

Cytokines interact with hematopoietin superfamily receptors and stimulate receptor dimerization. We demonstrate that chemoattractant cytokines (chemokines) also trigger biological responses through receptor dimerization. Functional responses are induced after pairwise crosslinking of chemokine receptors by bivalent agonistic antichemokine receptor mAb, but not by their Fab fragments. Monocyte chemoattractant protein (MCP)-1-triggered receptor dimerization was studied in human embryonic kidney (HEK)-293 cells cotransfected with genes coding for the CCR2b receptor tagged with YSK or Myc sequences. After MCP-1 stimulation, immunoprecipitation with Myc-specific antibodies revealed YSK-tagged receptors in immunoblotting. Receptor dimerization also was validated by chemical crosslinking in both HEK-293 cells and the human monocytic cell line Mono Mac 1. Finally, we constructed a loss-of-function CCR2bY139F mutant that acted as a dominant negative, blocking signalingthrough the CCR2 wild-type receptor. This study provides functional support for a model in which the MCP-1 receptor is activated by ligand-induced homodimerization, allowing discussion of the similarities between bacterial and leukocyte chemotaxis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 08:47:29