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Titolo:
Confocal calcium imaging reveals an ionotropic P2 nucleotide receptor in the paranodal membrane of rat Schwann cells
Autore:
Grafe, P; Mayer, C; Takigawa, T; Kamleiter, M; Sanchez-Brandelik, R;
Indirizzi:
Univ Munich, Dept Physiol, D-80336 Munich, Germany Univ Munich Munich Germany D-80336 Dept Physiol, D-80336 Munich, Germany
Titolo Testata:
OBSERVATORY
fascicolo: 1149, volume: 119, anno: 1999,
pagine: 377 - 383
SICI:
0029-7704(199904)119:1149<377:CCIRAI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
REGULATE INTRACELLULAR CALCIUM; MICROGLIAL CELLS; PURINERGIC RECEPTORS; ATP; RELEASE; P2X(7); TRANSIENTS; MOUSE; STIMULATION; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Grafe, P Univ Munich, Dept Physiol, Pettenkoferstr 12, D-80336 Munich, Germany Univ Munich Pettenkoferstr 12 Munich Germany D-80336 ch, Germany
Citazione:
P. Grafe et al., "Confocal calcium imaging reveals an ionotropic P2 nucleotide receptor in the paranodal membrane of rat Schwann cells", OBSERVATORY, 119(1149), 1999, pp. 377-383

Abstract

1. The paranodal Schwann cell region is of major importance for the function of a myelinated axon. In the present study we searched for a possible ionotropic effect of extracellular ATP in this Schwann cell compartment.2. Whole-cell patch-clamp recordings from cultured rat Schwann cells revealed that ATP and 2'-3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate (BzATP) induced a non-specific cation current. The effect of ATP was much enhanced in a Ca2+- and Mg2+ -free solution. ADP, UTP and alpha,beta-methylene adenosine 5'-triphosphate (alpha,beta-meATP) had no effect.3. Confocal Ca2+ imaging of myelinating; Schwann cells in isolated rat spinal roots showed a BzATP-induced rise in the free intracellular Ca2+ concentration in the paranodal Schwann cell cytoplasm whereas alpha,beta-meATP and 2-(methylthio)-adenosine 5'-triphosphate were without effect. In contrastto the known metabotropic effect of UTP on these Schwann cell regions, theBzATP-induced Ca2+ signal was not transient, was unaffected by depletion of intracellular Ca2+ stores and dependent on the presence of extracellular Ca2+.4. These results suggest that sin ionotropic ATP receptor with electrophysiological and pharmacological characteristics of the P2X(7) subtype of nucleotide receptors is functionally active in myelinating Schwann cells of peripheral nerves. Such a receptor might contribute to Schwann cell reactions in nerve injury or neuropathy.

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Documento generato il 24/11/20 alle ore 14:10:10