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Titolo:
Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution: Optical studies of nicotinic activity in an enteric plexus
Autore:
Obaid, AL; Koyano, T; Lindstrom, J; Sakai, T; Salzberg, BM;
Indirizzi:
Univ Penn, Sch Med, Dept Neurosci, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Neurosci, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Dept Physiol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 t Physiol, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 8, volume: 19, anno: 1999,
pagine: 3073 - 3093
SICI:
0270-6474(19990415)19:8<3073:SPOAIA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG ILEUM; CHOLINE-ACETYLTRANSFERASE-IMMUNOREACTIVITY; COOPERATIVE FIRING ACTIVITY; ACETYLCHOLINE-RECEPTORS; NERVOUS-SYSTEM; SYNAPTIC TRANSMISSION; MONOCLONAL-ANTIBODIES; ELECTRICAL-ACTIVITY; ULCERATIVE-COLITIS; SUBMUCOUS NEURONS;
Keywords:
optical recording; enteric nervous system; submucous plexus; nicotinic ACh receptors; voltage-sensitive dye; di-8-ANEPPS; phototoxicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Obaid, AL Univ4Penn, Sch Med, Dept Neurosci, 234 Stemmler Hall, Philadelphia, PA 1910 Univ Penn 234 Stemmler Hall Philadelphia PA USA 19104 a, PA 1910
Citazione:
A.L. Obaid et al., "Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution: Optical studies of nicotinic activity in an enteric plexus", J NEUROSC, 19(8), 1999, pp. 3073-3093

Abstract

Multiple Site Optical Recording of Transmembrane Voltage (MSORTV) has beenused to measure, continuously and simultaneously, the spontaneous electrical activity from all of the neurons in individual ganglia or up to five interconnected ganglia of the submucous plexus of the guinea pig small intestine. These are the first optical recordings of electrical activity with single-cell resolution from a mammalian nervous system. They are used to investigate the effects of acute and chronic application of nicotine on the firing patterns of this neural network containing important cholinergic components. After washout of acutely applied nicotine, the firing rates of selectedneurons were dramatically elevated. These results suggest that nAChRs thatreversibly desensitize after exposure to nicotine may be responsible for the enhancement of activity that is observed after a brief application of this agonist. In addition, immunostaining with monoclonal antibodies was usedto localize alpha 3/alpha 5, alpha 7, and beta 2 nAChR subunits, and the results demonstrate the prevalence of alpha 3/alpha 5. It is this alpha 3-containing nAChR subtype that probably accounts for most of the excess activity elicited by nicotine application.

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Documento generato il 09/04/20 alle ore 07:04:26