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Titolo:
Contrasting roles for nitric oxide and peroxynitrite in the peroxidation of myelin lipids
Autore:
van der Veen, RC; Roberts, LJ;
Indirizzi:
Univ So Calif, Sch Med, Dept Neurol, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 Neurol, Los Angeles, CA 90033 USA Vanderbilt Univ, Dept Med & Pharmacol, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Pharmacol, Nashville, TN 37232 USA
Titolo Testata:
JOURNAL OF NEUROIMMUNOLOGY
fascicolo: 1-2, volume: 95, anno: 1999,
pagine: 1 - 7
SICI:
0165-5728(19990301)95:1-2<1:CRFNOA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; LOW-DENSITY-LIPOPROTEIN; CELLULAR-ENERGY DEPLETION; CENTRAL-NERVOUS-SYSTEM; SMOOTH-MUSCLE CELLS; DNA STRAND BREAKAGE; SYNTHASE INHIBITORS; MULTIPLE-SCLEROSIS; RAT-BRAIN;
Keywords:
experimental allergic encephalomyelitis; myelin; isoprostanes; peroxynitrite; nitric oxide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: van der Veen, RC Univ,So Calif, Sch Med, Dept Neurol, 1333 San Pablo St,MCH 142, Los Angeles Univ So Calif 1333 San Pablo St,MCH 142 Los Angeles CA USA 90033
Citazione:
R.C. van der Veen e L.J. Roberts, "Contrasting roles for nitric oxide and peroxynitrite in the peroxidation of myelin lipids", J NEUROIMM, 95(1-2), 1999, pp. 1-7

Abstract

Peroxynitrite is formed by the reaction of nitric oxide (NO) and superoxide. Since widespread peroxynitrite activity was observed during experimentalallergic encephalomyelitis (EAE), the effect of this strong lipid-peroxidizing agent on myelin integrity was examined. Incubation of myelin suspensions with the peroxynitrite donor 3-morpholinosydnonimine (SIN-I) resulted inthe formation of the lipid peroxidation product, malondialdehyde (MDA). MDA formation was inhibited in the presence of butylated hydroxytoluene, which interrupts the progression of the lipid peroxidation chain reaction. Superoxide dismutase inhibited the effect of SIN-I, which indicates a role for superoxide, and contradicts a role for its dismutation product, hydrogen peroxide. The latter was confirmed by the failure of the catalase to inhibit MDA formation. Neither NO nor superoxide alone induced significant MDA formation in myelin, indicating that peroxynitrite formation is required for myelin-lipid peroxidation. Interestingly, NO actually inhibited lipid peroxidation in myelin, as demonstrated using simple NO donors. On the other hand,the simultaneous production of superoxide, as achieved with the NO-donor SIN-I, negated the inhibitory effect of NO. Finally, the production of isoprostanes, novel products generated during lipid peroxidation, was examined. Peroxynitrite-induced peroxidation of myelin resulted in isoprostane formation. Furthermore, increased levels of F-2-isoprostanes and neuroprostanes were observed in spinal cords of mice during early progressive stages of autoimmune encephalomyelitis. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 04/04/20 alle ore 14:40:24