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Titolo:
Lack of evolutionary stasis during alternating replication of an arbovirusin insect and mammalian cells
Autore:
Novella, IS; Hershey, CL; Escarmis, C; Domingo, E; Holland, JJ;
Indirizzi:
Med Coll Ohio, Dept Microbiol & Immunol, Toledo, OH 43614 USA Med Coll Ohio Toledo OH USA 43614 crobiol & Immunol, Toledo, OH 43614 USA Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 t Biol, La Jolla, CA 92093 USA Univ Calif San Diego, Inst Genet Mol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 et Mol, La Jolla, CA 92093 USA Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, Madrid 28049, SpainUniv Autonoma Madrid Madrid Spain 28049 evero Ochoa, Madrid 28049, Spain
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 3, volume: 287, anno: 1999,
pagine: 459 - 465
SICI:
0022-2836(19990402)287:3<459:LOESDA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICULAR STOMATITIS-VIRUS; RNA VIRUSES; FITNESS; GENOME; TRANSMISSION; RESISTANCE; SEQUENCES; PASSAGES; MUTANTS;
Keywords:
quasispecies; arbovirus; fitness; VSV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Novella, IS Med Coll Ohio, Dept Microbiol & Immunol, Hlth Educ Bldg 3055 Arlington Ave, Med Coll Ohio Hlth Educ Bldg 3055 Arlington Ave Toledo OH USA43614
Citazione:
I.S. Novella et al., "Lack of evolutionary stasis during alternating replication of an arbovirusin insect and mammalian cells", J MOL BIOL, 287(3), 1999, pp. 459-465

Abstract

The evolution of vesicular stomatitis virus (VSV) in a constant environment, consisting of either mammalian or insect cells, has been compared to theevolution of the same viral population in changing environments consistingin alternating passages in mammalian and insect cells. Fitness increases were observed in all cases. An initial fitness loss of VSV passaged in insect cells was noted when fitness was measured in BHK-21 cells, but this effect could be attributed to a difference of temperature during VSV replicationat 37 degrees C in BHK-21 cells. Sequencing of nucleotides 1-4717 at the 3' end of the VSV genome (N, P, M and G genes) showed that at passage 80 thenumber of mutations accumulated during alternated passages (seven mutations) is similar or larger than that observed in populations evolving in a constant environment (two to four mutations). Our results indicate that insectand mammalian cells can constitute similar environments for viral replication. Thus, the slow rates of evolution observed in natural populations of arboviruses are not necessarily due to the need for the virus to compromise between adaptation to both arthropod and vertebrate cell types. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 04:24:44