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Titolo:
The dependence for leukocyte function-associated antigen-1/ICAM-1 interactions in T cell activation cannot be overcome by expression of high density TCR ligand
Autore:
Abraham, C; Griffith, J; Miller, J;
Indirizzi:
Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 enet & Cell Biol, Chicago, IL 60637 USA Univ Chicago, Dept Med, Chicago, IL 60637 USA Univ Chicago Chicago IL USA60637 hicago, Dept Med, Chicago, IL 60637 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 8, volume: 162, anno: 1999,
pagine: 4399 - 4405
SICI:
0022-1767(19990415)162:8<4399:TDFLFA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR-ADHESION MOLECULE-1; PEPTIDE-MHC COMPLEXES; CLASS-II MOLECULES; ANTIGEN-RECEPTOR; COSTIMULATORY SIGNAL; MONOCLONAL-ANTIBODY; ACTIN CYTOSKELETON; INVARIANT CHAIN; FOCAL ADHESIONS; MINIMAL NUMBER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Miller, J UnivUSAicago, Dept Mol Genet & Cell Biol, 920 E 58Th St, Chicago, IL 60637 Univ Chicago 920 E 58Th St Chicago IL USA 60637 hicago, IL 60637
Citazione:
C. Abraham et al., "The dependence for leukocyte function-associated antigen-1/ICAM-1 interactions in T cell activation cannot be overcome by expression of high density TCR ligand", J IMMUNOL, 162(8), 1999, pp. 4399-4405

Abstract

The leukocyte-specific integrin, LFA-1, can enhance T cell activation. However, it is unclear whether the binding of LFA-1 to its ligand, ICAM-1, functions through intercellular adhesion alone, resulting in an augmentation of the TCR signal, or involves an additional LFA-l-mediated cellular signal transduction pathway. We have previously shown that naive CD4+ lymph node Tcells, isolated from DO11.10 TCR transgenic mice, are activated by increasing doses of exogenous OVA peptide presented by transfectants expressing both class II and ICAM-1, but not by cells expressing class II alone. To determine whether LFA-1/ICAM-1 interactions were simply enhancing the presentation of low concentrations of specific MHC/peptide complexes generated from exogenously added peptide, we transfected cells with class II that is covalently coupled to peptide, alone or in combination with ICAM-1. These cells express 100-fold more specific class II/peptide complexes than can be loaded onto class II-positive cells at maximum concentrations of exogenous peptide. Despite this high density of TCR ligand, activation of naive CD4+ T cells still requires the coexpression of ICAM-1, LFA-1/ICAM-1 interactions arenot required for effective conjugate formation and TCR engagement because presentation of class II/peptide complexes in the absence of ICAM-1 does induce up-regulation of CD25 and CD69, Thus, high numbers of engaged TCR cannot compensate for the lack of LFA-1/ICAM-1 interactions in the activation of naive CD4+ T cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:04:23