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Titolo:
Presence of the vesicular inhibitory amino acid transporter in GABAergic and glycinergic synaptic terminal boutons
Autore:
Dumoulin, A; Rostaing, P; Bedet, C; Levi, S; Isambert, MF; Henry, JP; Triller, A; Gasnier, B;
Indirizzi:
Inst Biol Physicochim, CNRS ERS 575, F-75005 Paris, France Inst Biol Physicochim Paris France F-75005 RS 575, F-75005 Paris, France Ecoles,ormale Super, Lab Biol Cellulaire Synapse, INSERM U497, F-75005 Pari Ecole Normale Super Paris France F-75005 apse, INSERM U497, F-75005 Pari
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 6, volume: 112, anno: 1999,
pagine: 811 - 823
SICI:
0021-9533(199903)112:6<811:POTVIA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC ACID; RAT SPINAL-CORD; IMMUNOREACTIVE NERVE-TERMINALS; CAENORHABDITIS-ELEGANS; ACETYLCHOLINE TRANSPORTER; MONOAMINE TRANSPORTER; MONOCLONAL-ANTIBODIES; GABA TRANSPORTER; ACTIVE-TRANSPORT; EXPRESSION;
Keywords:
neurotransmission; GABA; glycine; gephyrin; synaptic vesicle; neurotransmitter transporter; spinal cord; cerebellum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Gasnier, B Instaris, Physicochim, CNRS ERS 575, 13 Rue Pierre & Marie Curie, F-75005 P Inst Biol Physicochim 13 Rue Pierre & Marie Curie Paris France F-75005
Citazione:
A. Dumoulin et al., "Presence of the vesicular inhibitory amino acid transporter in GABAergic and glycinergic synaptic terminal boutons", J CELL SCI, 112(6), 1999, pp. 811-823

Abstract

The characterization of the Caenorhabditis elegans unc-47 gene recently allowed the identification of a mammalian gamma-amino butyric acid (GABA) transporter, presumed to be located in the synaptic vesicle membrane. In situ hybridization data in rat brain suggested that it might also take up glycine and thus represent a general Vesicular Inhibitory Amino Acid Transporter (VIAAT), In the present study, we have investigated the localization of VIAAT in neurons by using a polyclonal antibody raised against the hydrophilicN-terminal domain of the protein. Light microscopy and immunocytochemistryin primary cultures or tissue sections of the rat spinal cord revealed that VIAAT was localized in a subset (63-65%) of synaptophysin-immunoreactive terminal boutons; among the VIAAT-positive terminals around motoneuronal somata, 32.9% of them were also immunoreactive for GAD65, a marker of GABAergic presynaptic endings. Labelling was also found apposed to clusters positive for the glycine receptor or for its associated protein gephyrin, At the ultrastructural level, VIAAT immunoreactivity was restricted to presynapticboutons exhibiting classical inhibitory features and, within the boutons, concentrated over synaptic vesicle clusters. Pre-embedding detection of VIAAT followed by post-embedding detection of GABA or glycine on serial sections of the spinal cord or cerebellar cortex indicated that VIAAT was presentin glycine-, GABA- or GABA- and glycine-containing boutons, Taken together, these data further support the view of a common vesicular transporter forthese two inhibitory transmitters, which would be responsible for their costorage in the same synaptic vesicle and subsequent corelease at mixed GABA- and-glycine synapses.

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Documento generato il 30/03/20 alle ore 13:24:31