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Titolo:
Direct observations of the mechanical behaviors of the cytoskeleton in living fibroblasts
Autore:
Heidemann, SR; Kaech, S; Buxbaum, RE; Matus, A;
Indirizzi:
Michigan State Univ, Dept Physiol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 ysiol, E Lansing, MI 48824 USA Friedrich Miescher Inst, CH-4002 Basel, Switzerland Friedrich Miescher Inst Basel Switzerland CH-4002 002 Basel, Switzerland
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 1, volume: 145, anno: 1999,
pagine: 109 - 122
SICI:
0021-9525(19990405)145:1<109:DOOTMB>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROTUBULE-ASSOCIATED PROTEINS; AXONAL ELONGATION; ANIMAL-CELLS; ACTIN; CYTOMECHANICS; TENSION; REORGANIZATION; CONTRACTION; PLASTICITY; DEPENDENCE;
Keywords:
cytoskeleton; cytomechanics; biorheology; integrins; cell shape;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Heidemann, SR Michigan State Univ, Dept Physiol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 ng, MI 48824 USA
Citazione:
S.R. Heidemann et al., "Direct observations of the mechanical behaviors of the cytoskeleton in living fibroblasts", J CELL BIOL, 145(1), 1999, pp. 109-122

Abstract

Cytoskeletal proteins tagged with green fluorescent protein were used to directly visualize the mechanical Pole of the cytoskeleton in determining cell shape. Rat embryo (REF 52) fibroblasts were deformed using glass needleseither uncoated for purely physical manipulations, or coated with laminin to induce attachment to the cell surface. Cells responded to uncoated probes in accordance with a three-layer model in which a highly elastic nucleus is surrounded by cytoplasmic microtubules that behave as a jelly-like viscoelastic fluid. The third, outermost cortical layer is an elastic shell under sustained tension. Adhesive, laminin-coated needles caused focal recruitment of actin filaments to the contacted surface region and increased the cortical layer stiffness. This direct visualization of actin recruitment confirms a widely postulated model for mechanical connections between extracellular matrix proteins and the actin cytoskeleton. Cells tethered to laminin-treated needles strongly resisted elongation by actively contracting. Whether using uncoated probes to apply simple deformations or laminin-coated probes to induce surface-to-cytoskeleton interaction we observed that experimentally applied forces produced exclusively local responses by both the actin and microtubule cytoskeleton. This local accomodation and dissipation of force is inconsistent with the proposal that cellular tensegrity determinescell shape.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 06:46:12