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Titolo:
Specific components of the SAGA complex are required for Gcn4- and Gcr1-mediated activation of the his4-912 delta promoter in Saccharomyces cerevisiae
Autore:
Dudley, AM; Gansheroff, LJ; Winston, F;
Indirizzi:
Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ch Med, Dept Genet, Boston, MA 02115 USA
Titolo Testata:
GENETICS
fascicolo: 4, volume: 151, anno: 1999,
pagine: 1365 - 1378
SICI:
0016-6731(199904)151:4<1365:SCOTSC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
START-SITE SELECTION; RNA-POLYMERASE-II; TATA-BINDING PROTEIN; TRANSCRIPTION INITIATION SITES; TRANSPOSABLE ELEMENT TY1; ACID BIOSYNTHETIC GENES; HIS4 TRANSCRIPTION; EXPRESSION REQUIRES; CHROMATIN STRUCTURE; ZINC-FINGER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
75
Recensione:
Indirizzi per estratti:
Indirizzo: Winston, F Harvard Univ, Sch Med, Dept Genet, 200 Longwood Ave, Boston, MA02115 USA Harvard Univ 200 Longwood Ave Boston MA USA 02115 MA 02115 USA
Citazione:
A.M. Dudley et al., "Specific components of the SAGA complex are required for Gcn4- and Gcr1-mediated activation of the his4-912 delta promoter in Saccharomyces cerevisiae", GENETICS, 151(4), 1999, pp. 1365-1378

Abstract

Mutations selected as suppressors of Ty or solo delta insertion mutations in Saccharomyces cerevisiae have identified several genes, SPT3, SPT7, SPT8, and SPT20, that encode components of the SAGA complex. However, the mechanism by which SAGA activates transcription of specific RNA polymerase II-dependent genes is unknown. We have conducted a fine-structure mutagenesis ofone widely used SAGA-dependent promoter, the delta element of his4-912 delta, to identify sequence elements important for its promoter activity. Our analysis has characterized three delta regions necessary for full promoter activity and accurate start site selection: an upstream activating sequence, a TATA region, and an initiator region. In addition, we have shown that factors present at the adjacent UAS(HIS4) (Gcn4, Bas1, and Pho2) also activate the delta promoter in his4-912 delta. Our results suggest a model in which the delta promoter in his4-912 delta is primarily activated by two factors: Gcr1 acting at the UAS(delta) and Gcn4 acting at the UAS(HIS4). Finally, we tested whether activation by either of these factors is dependent on components of the SAGA complex. Our results demonstrate that Spt3 and Spt20 are required for full delta promoter activity, but that Gcn5, another member of SAGA, is not required. Spt3 appears to be partially required for activation of his4-912 delta by both Gcr1 and Gcn4. Thus, our work suggests thatSAGA exerts a large effect on delta promoter activity through a combination of smaller effects on multiple factors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 22:16:29