Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization of GAPCenA, a GTPase activating protein for Rab6, part ofwhich associates with the centrosome
Autore:
Cuif, MH; Possmayer, F; Zander, H; Bordes, N; Jollivet, F; Couedel-Courteille, A; Janoueix-Lerosey, I; Langsley, G; Bornens, M; Goud, B;
Indirizzi:
Inst Curie, UMR CNRS 144 & 168, F-75248 Paris 05, France Inst Curie Paris France 05 UMR CNRS 144 & 168, F-75248 Paris 05, France Inst Pasteur, Dept Immunol, CNRS, URA 1960, F-75724 Paris, France Inst Pasteur Paris France F-75724 CNRS, URA 1960, F-75724 Paris, France
Titolo Testata:
EMBO JOURNAL
fascicolo: 7, volume: 18, anno: 1999,
pagine: 1772 - 1782
SICI:
0261-4189(19990401)18:7<1772:COGAGA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPINDLE POLE BODY; GAMMA-TUBULIN; BINDING PROTEIN; CELL-CYCLE; SCHIZOSACCHAROMYCES-POMBE; SACCHAROMYCES-CEREVISIAE; MICROTUBULE ORGANIZATION; ENDOPLASMIC-RETICULUM; NUCLEOTIDE EXCHANGE; SEPTUM FORMATION;
Keywords:
centrosome; Golgi apparatus; GTPase activating protein; mitosis; Rab6;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Goud, B Inst Curie, UMR CNRS 144 & 168, 26 Rue Ulm, F-75248 Paris 05, France Inst Curie 26 Rue Ulm Paris France 05 m, F-75248 Paris 05, France
Citazione:
M.H. Cuif et al., "Characterization of GAPCenA, a GTPase activating protein for Rab6, part ofwhich associates with the centrosome", EMBO J, 18(7), 1999, pp. 1772-1782

Abstract

The Rab6 GTPase regulates intracellular transport at the level of the Golgi apparatus, probably in a retrograde direction. Here, we report the identification and characterization of a novel human Rab6-interacting protein named human GAPCenA (for 'GAP and centrosome-associated'). Primary sequence analysis indicates that GAPCenA displays similarities, within a central 200 amino acids domain, to both the yeast Rab GTPase activating proteins (GAPs) and to the spindle checkpoint proteins Saccharomyces cerevisiae Bub2p and Schizosaccharomyces pombe Cdc16p, We demonstrate that GAPCenA is indeed a CAP, specifically active in vitro On Rab6 and, to a lesser extent, on Rab4 and Rab2, proteins. Immunofluorescence and cell fractionation experiments showed that GAPCenA is mainly cytosolic but that a minor pool is associated with the centrosome, Moreover, GAPCenA was found to form complexes with cytosolic gamma-tubulin and to play a role in microtubule nucleation. Therefore,GAPCenA may be involved in the coordination of microtubule and Colgi dynamics during the cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:58:06