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Titolo:
Peaks in capillary zone electrophoresis: Fact or fiction
Autore:
Beckers, J; Bocek, P;
Indirizzi:
Acad Sci Czech Republ, Inst Analyt Chem, CS-61142 Brno, Czech Republic Acad Sci Czech Republ Brno Czech Republic CS-61142 Brno, Czech Republic Eindhovenlands Technol, Instrumental Anal Lab, NL-5600 MB Eindhoven, Nether Eindhoven Univ Technol Eindhoven Netherlands NL-5600 MB indhoven, Nether
Titolo Testata:
ELECTROPHORESIS
fascicolo: 3, volume: 20, anno: 1999,
pagine: 518 - 524
SICI:
0173-0835(199903)20:3<518:PICZEF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDIRECT UV DETECTION; SAMPLE ZONES; SYSTEM PEAKS; QUANTIFICATION; ANIONS;
Keywords:
system peak; capillary zone electrophoresis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Bocek, P Acadpubliczech Republ, Inst Analyt Chem, Veveri 97, CS-61142 Brno, Czech Re Acad Sci Czech Republ Veveri 97 Brno Czech Republic CS-61142 Re
Citazione:
J. Beckers e P. Bocek, "Peaks in capillary zone electrophoresis: Fact or fiction", ELECTROPHOR, 20(3), 1999, pp. 518-524

Abstract

Non-steady-state electrophoretic processes can be estimated by a repeated application of a steady-state model based on the electroneutrality equation, the modified version of Ohm's law, and the mass balances of the co- and counterions. With such a mathematical model, all parameters in sample zones in capillary zone electrophoresis (CZE) can be calculated. The relationships between the calculated parameters for sample zones in CZE, such as the pH, concentrations of co- and counterions, and the ratio E(1)m(1)/E(2)m(2) versus the mobilities of both anionic and cationic analytes can be visualizedin a SystChart, a set of eight panels, for a given background electrolyte (BGE). All properties of a zone, such as the fronting/tailing character andthe question of peaks/dips can be read from such a SystChart. Applying n colons, n-1 discontinuities are present in such a SystChart, indicating the presence of system peaks applying that BGE. For BGEs with one colon, no system peaks (discontinuities) exist at moderate pH values. SystCharts calculated for BGEs with a low pH do show discontinuities, however, which indicates that system peaks are present in electropherograms applying BGEs at low pH. Experimentally, it is shown that system peaks are indeed present in electropherograms applying BGEs with one colon at low pH and the mobilities of the system peaks generally increase with decreasing pH. Hydrogen ions seem to act as a second coionic species. Of course, these system peaks are only visible in the UV signal if the BGE has UV-absorbing properties.

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Documento generato il 04/07/20 alle ore 20:19:01