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Titolo:
Mass spectrometric analysis of the electroeluates of fluorescent proteins after preparative electrophoresis in the automated HPGE-1000 apparatus
Autore:
Yarmola, E; Chrambach, A; Nguyen, VQ; Yergey, AL;
Indirizzi:
NICHD,92acromol Anal Sect, Lab Cellular & Mol Biphys, NIH, Bethesda, MD 208 NICHD Bethesda MD USA 20892 Cellular & Mol Biphys, NIH, Bethesda, MD 208 Russianssiad Sci, Lab Biopolymer Phys, Engelhardt Inst Mol Biol, Moscow, Ru Russian Acad Sci Moscow Russia ys, Engelhardt Inst Mol Biol, Moscow, Ru NICHD,hesda,Mass Spectrometry & Metab, Lab Cellular & Mol Biophys, NIH, Bet NICHD Bethesda MD USA etry & Metab, Lab Cellular & Mol Biophys, NIH, Bet
Titolo Testata:
ELECTROPHORESIS
fascicolo: 3, volume: 20, anno: 1999,
pagine: 445 - 451
SICI:
0173-0835(199903)20:3<445:MSAOTE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBCELLULAR-SIZED PARTICLES; GEL-ELECTROPHORESIS; POLYMER-SOLUTION; SDS-PROTEIN; SEPARATION; RECOVERY; DNA;
Keywords:
mass spectrometry; fluorescent proteins; electroeluates; automated gel electrophoresis with periodic fluorescence scanning apparatus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Chrambach, A NICHD,0,acromol Anal Sect, Lab Cellular & Mol Biphys, NIH, Bldg 10,Room 9D5 NICHD Bldg 10,Room 9D50 Bethesda MD USA 20892 ldg 10,Room 9D5
Citazione:
E. Yarmola et al., "Mass spectrometric analysis of the electroeluates of fluorescent proteins after preparative electrophoresis in the automated HPGE-1000 apparatus", ELECTROPHOR, 20(3), 1999, pp. 445-451

Abstract

Bands of green fluorescent protein (GFP) and R-phycoerythrin (PHYCO) in gel electrophoresis on the automated apparatus for gel electrophoresis with periodic fluorescence scanning (HPGE), the HPGE-1000 apparatus, were retrieved from the gel by electroelution. While PHYCO was recovered in a single volume of electroeluate buffer after the predicted migration time, GFP fluorescence was lost under the same conditions and could only be recovered usingmultiple changes or electroeluate buffer. The multiple volumes of buffer necessitated pooling, concentration, and storage, conditions under which a minor GFP component, GFP-II, formed artifactually. PHYCO after electroelution also exhibits a minor component present in the original preparation. The electroeluate of GFP, transferred into a mass spectrometer after pooling, concentration and storage, is indistinguishable in mass from the original preparation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:09:55