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Titolo:
Cytofluorometry and fluorescence confocal laser scanning microscopy (CLSM)in the study of neutral lipid dynamics in Paramecium primaurelia mating types during cell line development
Autore:
Ramoino, P; Diaspro, A; Fato, M; Beltrame, F;
Indirizzi:
Univ Genoa, Ist Zool, I-16126 Genoa, Italy Univ Genoa Genoa Italy I-16126 niv Genoa, Ist Zool, I-16126 Genoa, Italy INFM, Genoa, Italy INFM Genoa ItalyINFM, Genoa, Italy Univ Genoa, Dept Phys, Genoa, Italy Univ Genoa Genoa ItalyUniv Genoa, Dept Phys, Genoa, Italy Univ Genoa, Dept Commun Comp & Syst Sci, I-16145 Genoa, Italy Univ Genoa Genoa Italy I-16145 mun Comp & Syst Sci, I-16145 Genoa, Italy
Titolo Testata:
CYTOMETRY
fascicolo: 4, volume: 35, anno: 1999,
pagine: 346 - 352
SICI:
0196-4763(19990401)35:4<346:CAFCLS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NILE RED; CULTURE AGE; PROBE;
Keywords:
nile red; lipid droplets; clonal development; culture growth; phagocytosis; confocal laser scanning microscopy; ciliated protozoa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Ramoino, P Univ Genoa, Ist Zool, Via Balbi 5, I-16126 Genoa, Italy Univ Genoa Via Balbi 5 Genoa Italy I-16126 -16126 Genoa, Italy
Citazione:
P. Ramoino et al., "Cytofluorometry and fluorescence confocal laser scanning microscopy (CLSM)in the study of neutral lipid dynamics in Paramecium primaurelia mating types during cell line development", CYTOMETRY, 35(4), 1999, pp. 346-352

Abstract

Background: In Paramecium primaurelia, an exconjugant cell can produce twoLines with different mating capacities. Mating type II cells can form a higher food vacuole number and digest the nutrient taken up in a shorter time; thus, mating type II cells grow at a faster rate than do mating type I cells. The present study was done to determine whether cells that ingest morenutrients also have a larger amount of storage lipids. Methods: Quantitative and qualitative determinations of neutral lipids were obtained by means of cytofluorometry and fluorescence confocal laser scanning microscopy (CLSM), respectively, by using nile red on cells in different physiologic states. Results: Lipid droplet number and neutral lipid content were higher in mating type II cells than in mating type I cells in the early logarithmic growth phase (i.e., immature well-fed cells). These values were reversed duringthe middle and the late logarithmic phases and became equal in the stationary phase (i.e., mature starved cells). In well-fed cells maintained with food excess, differences in neutral lipid content between the two mating types also were present in mature cells. Conclusions: Although differences between mating type I and mating type IIlines were not correlated to cell size, a relation was found between lipidcontent and food ingestion capacity. A depletion of bacteria in the culture medium could be responsible for the lack of differences in mature starvedcells. CLSM allowed us to gather volume information about the lipid droplet distribution within the cell. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:11:12