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Titolo:
The effect of bile salts and calcium on isolated rat liver mitochondria
Autore:
Guldutuna, S; Zimmer, G; Leuschner, M; Bhatti, S; Elze, A; Deisinger, B; Hofmann, M; Leuschner, U;
Indirizzi:
Univrankfurt,t, Ctr Internal Med, Dept Gastroenterol, Med Clin 2, D-60590 F Univ Frankfurt Frankfurt Germany D-60590 oenterol, Med Clin 2, D-60590 F
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
fascicolo: 3, volume: 1453, anno: 1999,
pagine: 396 - 406
SICI:
0925-4439(19990330)1453:3<396:TEOBSA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMARY BILIARY-CIRRHOSIS; ELECTRON-TRANSPORT CHAIN; URSODEOXYCHOLIC ACID; GLUTAMATE-DEHYDROGENASE; TAUROURSODEOXYCHOLIC ACID; CHENODEOXYCHOLIC ACID; ILEAL ABSORPTION; MEMBRANE DAMAGE; CYTOSOLIC CA2+; HEPATOCYTES;
Keywords:
ursodeoxycholic acid; chenodeoxycholic acid; enzyme release; mitochondrion; membrane structure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Leuschner, U Univternnkfurt, Ctr Internal Med, Dept Gastroenterol, Med Clin 2, Theodor S Univ Frankfurt Theodor Stern Kai 7 Frankfurt Germany D-60590
Citazione:
S. Guldutuna et al., "The effect of bile salts and calcium on isolated rat liver mitochondria", BBA-MOL BAS, 1453(3), 1999, pp. 396-406

Abstract

Intact mitochondria were incubated with and without calcium in solutions of chenodeoxycholate, ursodeoxycholate, or their conjugates. Glutamate dehydrogenase, protein and phospholipid release were measured. Alterations in membrane and organelle structure were investigated by electron paramagnetic resonance spectroscopy. Chenodeoxycholate enhanced enzyme liberation, solubilized protein and phospholipid, and increased protein spin label mobility and the polarity of the hydrophobic membrane interior, whereas ursodeoxycholate and its conjugates did not damage mitochondria. Preincubation with ursodeoxycholate or its conjugate tauroursodeoxycholate for 20 min partially prevented damage by chenodeoxycholate. Extended preincubation even with 1 mM ursodeoxycholate could no longer prevent structural damage. Calcium (from 0.01 mM upward) augmented the damaging effect of chenodeoxycholate (0.15-0.5mM). The combined action of 0.01 mM calcium and 0.15 mM chenodeoxycholate was reversed by ursodeoxycholate only, not by its conjugates tauroursodeoxycholate and glycoursodeoxycholate. In conclusion, ursodeoxycholate partially prevents chenodeoxycholate-induced glutamate dehydrogenase release from liver cell mitochondria by membrane stabilization. This holds for shorter times and at concentrations below 0.5 mM only, indicating that the different constitution of protein-rich mitochondrial membranes does not allow optimalstabilization such as has been seen in phospholipid- and cholesterol-rich hepatocyte cell membranes, investigated previously. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 07:06:26