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Titolo:
Adhesive interaction between P-selectin and sialyl Lewis(x) plays an important role in recurrent coronary arterial thrombosis in dogs
Autore:
Ikeda, H; Ueyama, T; Murahara, T; Yasukawa, H; Haramaki, N; Eguchi, H; Katoh, A; Takajo, Y; Onitsuka, I; Ueno, T; Tojo, SJ; Imaizumi, T;
Indirizzi:
Kurume Univ, Sch Med, Dept Internal Med 3, Kurume, Fukuoka 830, Japan Kurume Univ Kurume Fukuoka Japan 830 al Med 3, Kurume, Fukuoka 830, Japan Sumitomo Pharmaceut Co Ltd, Res Ctr, Osaka, Japan Sumitomo Pharmaceut Co Ltd Osaka Japan ut Co Ltd, Res Ctr, Osaka, Japan
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 4, volume: 19, anno: 1999,
pagine: 1083 - 1090
SICI:
1079-5642(199904)19:4<1083:AIBPAS>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC FLOW VARIATIONS; GRANULE MEMBRANE-PROTEIN; MYOCARDIAL REPERFUSION INJURY; PLATELET-AGGREGATION; MONOCLONAL-ANTIBODY; ACTIVATED PLATELETS; NEUTROPHIL ADHESION; ENDOTHELIAL INJURY; UNSTABLE ANGINA; CELL-ADHESION;
Keywords:
thrombosis; platelets; leukocytes; P-selectin; sialyl Lewis(x);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Ikeda, H Kurume830,v, Sch Med, Dept Internal Med 3, 67 Asahi Machi, Kurume, Fukuoka Kurume Univ 67 Asahi Machi Kurume Fukuoka Japan 830 rume, Fukuoka
Citazione:
H. Ikeda et al., "Adhesive interaction between P-selectin and sialyl Lewis(x) plays an important role in recurrent coronary arterial thrombosis in dogs", ART THROM V, 19(4), 1999, pp. 1083-1090

Abstract

Cell adhesion molecules may play an important role in the disease process of acute coronary syndromes. We have shown a neutralizing anti-P-selectin monoclonal antibody and a sialyl Lewis(x)-containing oligosaccharide (SLe(x)-OS), an analogue of selectin ligand on leukocytes, reduce cyclic flow variations (CFVs) in a canine model of recurrent coronary arterial thrombosis, suggesting the important interaction between P-selectin and SLe(x) for the pathophysiology of these syndromes. However, the functional role of these adhesion molecules in the thrombotic process remains unclear. Therefore, we investigated effects of SLe(x)-OS on CFVs, platelet P-selectin expression, and morphology of the stenotic site in the same model. Anesthetized open-chest dogs (n=34) were randomly divided into 4 groups after developing CFVs. Dogs intravenously received saline or graded doses of SLe(x)-OS (5, 20, or 40 mg/kg bolus) infusion followed by a continuous infusion (5 mg . kg(-1) .h(-1)) for 60 minutes. By flow cytometric analysis, P-selectin expression on platelets after CFVs was significantly upregulated during CFVs. Immunohistochemical analysis revealed the incorporation of platelets with upregulated P-selectin within thrombi at the stenotic site. Microscopic observationsrevealed the presence of numerous platelets adhered to leukocytes at the stenotic site on the damaged endothelium. SLe(x)-OS significantly reduced CFVs, inhibited the P-selectin expression on platelets, and prevented the adherence of platelets and leukocytes. These findings further support the notion that the adhesive interaction between P-selectin on platelets and SLe(x)on leukocytes plays an important role in platelet-mediated thrombus formation in this model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:16:52