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Titolo:
The use of quantitative PCR, plant and earthworm bioassays, plating and chemical analysis to monitor 4-chlorobiphenyl biodegradation in soil microcosms
Autore:
Ducrocq, V; Pandard, P; Hallier-Soulier, S; Thybaud, E; Truffaut, N;
Indirizzi:
Univ Technol Compiegne, Lab Gen Microbienne, F-60205 Compiegne, France Univ Technol Compiegne Compiegne France F-60205 -60205 Compiegne, France INERIS, Lab Ecotoxicol, F-60550 Verneuil Halatte, France INERIS Verneuil Halatte France F-60550 F-60550 Verneuil Halatte, France
Titolo Testata:
APPLIED SOIL ECOLOGY
fascicolo: 1, volume: 12, anno: 1999,
pagine: 15 - 27
SICI:
0929-1393(199904)12:1<15:TUOQPP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; POLYCHLORINATED-BIPHENYLS; CONTAMINATED SOILS; BPH OPERON; DEGRADATION; BACTERIA; GENES; BIOREMEDIATION; EXTRACTION; SURVIVAL;
Keywords:
4-chlorobiphenyl; biodegradation; soil microcosms; quantitative PCR; ecotoxicology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Truffaut, N Univncechnol Compiegne, Lab Gen Microbienne, BP 529, F-60205 Compiegne, Fra Univ Technol Compiegne BP 529 Compiegne France F-60205 e, Fra
Citazione:
V. Ducrocq et al., "The use of quantitative PCR, plant and earthworm bioassays, plating and chemical analysis to monitor 4-chlorobiphenyl biodegradation in soil microcosms", APPL SOIL E, 12(1), 1999, pp. 15-27

Abstract

4-Chlorobiphenyl biodegradation was monitored using several methods including molecular detection, microbiological and ecotoxicological techniques and analytical tools. Experiments were carried out in microcosms with artificial soil and an uncultivated sandy loam soil, contaminated at a rate of 1 gkg(-1) of 4-chlorobiphenyl. Two strains were used for bioremediation: a 4-chlorobiphenyl degrader, the bacterial strain Pseudomonas sp, strain B4 andthe bacterial strain Pseudomonas sp, strain CBS3 which is able to mineralize 4-chlorobenzoate. Molecular tools were constructed for the detection andthe quantification of dihydroxybiphenyl dioxygenase gene (bphC) from Pseudomonas sp. strain B4 and for the detection of the 4-chlorobenzoate coenzymeA dehalogenase gene from Pseudomonas sp. strain CBS3. Evidence was found for 4-chlorobiphenyl toxicity to barley and earthworms using standardized ecotoxicological tests. When Pseudomonas sp. strain B4 was introduced alone into microcosms, no decrease in toxicity occurred although 4-chlorobiphenyl was entirely degraded. The introduction of Pseudomonas sp. strain CBS3 intoinoculated microcosms resulted in the disappearance of the 4-chlorobenzoate accumulated during the degradation of 4-chlorobiphenyl by Pseudomonas sp.strain B4, and in the disappearance of any toxic effect indicating that remediation was complete. Similar results were obtained independent of the nature of the soil used in microcosms. These results underline the need for taking biological effects into account in order to assess remediation efficiency. (C) 1999 Elsevier Science B.V, All rights reserved.

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Documento generato il 04/12/20 alle ore 13:13:35